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Typical Testing Data/Standard Curve (for reference only)

Rat Troponin I Type 1, Slow Skeletal (TNNI1) ELISA Kit | TNNI1 elisa kit

Rat Troponin I Type 1, Slow Skeletal (TNNI1) ELISA Kit

Gene Names
TNNI1; TNN1; SSTNI
Reactivity
Rat
Synonyms
Troponin I Type 1; Slow Skeletal (TNNI1); Rat Troponin I Type 1; Slow Skeletal (TNNI1) ELISA Kit; TNN1; Troponin I; slow-twitch isoform; TNNI1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Troponin I Type 1, Slow Skeletal (TNNI1).
No significant cross-reactivity or interference between Troponin I Type 1, Slow Skeletal (TNNI1) and analogues was observed.
Sequence Length
187
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
2.50-160ng/mL
Sensitivity
< 0.75ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Troponin I Type 1, Slow Skeletal (TNNI1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Troponin I Type 1, Slow Skeletal (TNNI1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNNI1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 1, Slow Skeletal (TNNI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 1, Slow Skeletal (TNNI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TNNI1 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,692 Da
NCBI Official Full Name
Troponin I type 1 (skeletal, slow)
NCBI Official Synonym Full Names
troponin I type 1 (skeletal, slow)
NCBI Official Symbol
TNNI1
NCBI Official Synonym Symbols
TNN1; SSTNI
NCBI Protein Information
troponin I, slow skeletal muscle
UniProt Protein Name
Troponin I, slow skeletal muscle
Protein Family
UniProt Gene Name
TNNI1
UniProt Entry Name
TNNI1_HUMAN

NCBI Description

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: troponin complex; cytosol

Molecular Function: metal ion binding; actin binding

Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding

Research Articles on TNNI1

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Product Notes

The Rat TNNI1 tnni1 (Catalog #AAA2019567) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019567 ELISA Kit recognizes Rat TNNI1. It is sometimes possible for the material contained within the vial of "Troponin I Type 1, Slow Skeletal (TNNI1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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