Rat Dopamine Receptor D1 (DRD1) ELISA Kit | DRD1 elisa kit

Rat Dopamine Receptor D1 (DRD1) ELISA Kit

Cat. Num. AAA2021347

• Gene Names: DRD1; DADR; DRD1A
• Reactivity: Rat
• Synonyms: Dopamine Receptor D1 (DRD1); Rat Dopamine Receptor D1 (DRD1) ELISA Kit; DR-D1; D1R; DADR; DRD1A; DRD1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dopamine Receptor D1 (DRD1).; No significant cross-reactivity or interference between Dopamine Receptor D1 (DRD1) and analogues was observed.
• Sequence Length: 446

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Receptor D1 (DRD1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Receptor D1 (DRD1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DRD1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D1 (DRD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D1 (DRD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D1 (DRD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Receptor D1 (DRD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DRD1 elisa kit

Signal transduction; Cardiovascular biology; Neuroscience; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 20269979
• NCBI GeneID: 1812
• UniProt Accession #: P21728; Q4QRJ0; B2RA44
• Molecular Weight: 49,293 Da
• NCBI Official Full Name: dopamine receptor D1
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: DRD1
• NCBI Official Synonym Symbols: DADR; DRD1A
• NCBI Protein Information: D(1A) dopamine receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: DRD1
• UniProt Entry Name: DRD1_HUMAN

NCBI Description

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: protein binding; dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity

Biological Process: synaptic transmission, dopaminergic; peristalsis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; prepulse inhibition; positive regulation of potassium ion transport; generation of action potential; thermoregulation; adult walking behavior; dopamine metabolic process; positive regulation of synaptic transmission, glutamatergic; vasodilation; G-protein signaling, adenylate cyclase activating pathway; synaptogenesis; behavioral response to cocaine; protein import into nucleus; conditioned taste aversion; positive regulation of cAMP biosynthetic process; visual learning; dopamine receptor, adenylate cyclase activating pathway; response to drug; grooming behavior; dopamine transport; dentate gyrus development; striatum development; cerebral cortex GABAergic interneuron migration; maternal behavior; adenylate cyclase activation; regulation of dopamine metabolic process; response to amphetamine; mating behavior; regulation of dopamine uptake; transmission of nerve impulse; astrocyte development; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; habituation; glucose import; sensitization; operant conditioning; dopamine receptor, phospholipase C activating pathway; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cell migration