Human Acylglycerol Kinase (AGK) ELISA Kit | AGK elisa kit

Human Acylglycerol Kinase (AGK) ELISA Kit

Cat. Num. AAA2020177

• Gene Names: AGK; MULK; CATC5; CTRCT38; MTDPS10
• Reactivity: Human
• Synonyms: Acylglycerol Kinase (AGK); Human Acylglycerol Kinase (AGK) ELISA Kit; MULK; Multiple Substrate Lipid Kinase; AGK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Acylglycerol Kinase (AGK).; No significant cross-reactivity or interference between Acylglycerol Kinase (AGK) and analogues was observed.
• Sequence Length: 422

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.081ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acylglycerol Kinase (AGK) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acylglycerol Kinase (AGK) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGK elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acylglycerol Kinase (AGK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acylglycerol Kinase (AGK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acylglycerol Kinase (AGK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acylglycerol Kinase (AGK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AGK elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 8922701
• NCBI GeneID: 55750
• NCBI Accession #: NP_060708.1
• NCBI GenBank Nucleotide #: NM_018238.3
• UniProt Accession #: Q53H12; Q75KN1; Q96GC3; Q9NP48
• Molecular Weight: 7,634 Da
• NCBI Official Full Name: acylglycerol kinase, mitochondrial
• NCBI Official Synonym Full Names: acylglycerol kinase
• NCBI Official Symbol: AGK
• NCBI Official Synonym Symbols: MULK; CATC5; CTRCT38; MTDPS10
• NCBI Protein Information: acylglycerol kinase, mitochondrial
• UniProt Protein Name: Acylglycerol kinase, mitochondrial
• Protein Family: Acylglycerol kinase
• UniProt Gene Name: AGK
• UniProt Synonym Gene Names: MULK; hAGK; HsMuLK; MuLK; Multi-substrate lipid kinase
• UniProt Entry Name: AGK_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]

Uniprot Description

AGK: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. Defects in AGK are the cause of mitochondrial DNA depletion syndrome type 10 (MTDPS10). An autosomal recessive mitochondrial disorder characterized by congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, exercise intolerance, and lactic acidosis. Mental development is normal, but affected individuals may die early from cardiomyopathy. Defects in AGK are the cause of cataract, congenital, autosomal recessive type 5 (CATC5). CATC5 consists of an opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; EC 2.7.1.107; Kinase, lipid; EC 2.7.1.94

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: intracellular membrane-bound organelle; mitochondrion; mitochondrial membrane

Molecular Function: acylglycerol kinase activity; ceramide kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: protein kinase C activation; glycerolipid metabolic process; ceramide biosynthetic process; lipid phosphorylation

Disease: Cataract 38; Sengers Syndrome