Rabbit GANP Polyclonal Antibody | anti-GANP antibody

GANP (E1872) Polyclonal Antibody

Cat. Num. AAA3000543

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GANP; Polyclonal Antibody; GANP (E1872) Polyclonal Antibody; Germinal-center associated nuclear protein; 80 kDa MCM3-associated protein; MCM3 acetylating protein; MCM3 acetyltransferase; MCM3AP; KIAA0572; MAP80; anti-GANP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GANP (E1872) polyclonal antibody detects endogenous levels of GANP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1980

• Applicable Applications for anti-GANP antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1841-1890 of Human GANP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of GANP (E1872) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-GANP antibody

Minichromosome maintenance protein 3 (MCM3) is a protein essential for the initiation of DNA replication. MCM3-associated protein (MCM3AP), also known as GANP or MAP80, is a 1980 amino acid protein which binds to MCM3. MCM3AP is an acetyltransferase that acetylates MCM3, thereby playing an indirect role in DNA replication. MCM3AP is also believed to be involved in the nuclear localization of MCM3. Localized to the nucleus and the cytoplasm, GANP (MCM3AP) is thought to have phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions. MCM3AP contains a nuclear localization signal, a coiled-coil structure and four LXXLL motifs.

NCBI and Uniprot Product Information

• NCBI GI #: 19923191
• NCBI Accession #: NP_003897.2
• NCBI GenBank Nucleotide #: NP_003897.2
• UniProt Accession #: O60318
• Molecular Weight: ~ 218kDa
• NCBI Official Full Name: germinal-center associated nuclear protein
• UniProt Protein Name: 80 kDa MCM3-associated protein
• Protein Family: Putative arabinogalactan oligomer transport system permease protein
• UniProt Gene Name: MCM3AP
• UniProt Synonym Gene Names: GANP; KIAA0572; MAP80
• UniProt Entry Name: MCM3A_HUMAN

Uniprot Description

GANP: is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3¿ region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-¿B, and p53 consensus transcription factor binding sites are located. In contrast, the GANP promoter region contains consensus transcription factor binding sites for E2F, E-box, BSAP, and PU.1. MCM3AP interacts with MCM3, which it can acetylate weakly in vivo. GANP is required for efficient mRNA nuclear export: GANP depletion results in the nuclear accumulation of poly(A)+RNA. It interacts via its N-terminal FG repeat domain with the mRNA export factor NXF1. GANP plays a role in the immune response: antibodies from mice deficient for GANP have reduced affinities against T-cell dependent antigens and a lower frequency of variable region somatic mutation. GANP expression is markedly decreased in malignant glioma (MG) with a poor prognosis, consistently lower than that observed in anaplastic astrocytomas. Significant decreases in GANP expression are associated with hyperploidy and chromosomal instability

Protein type: Acetyltransferase; Karyopherin

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nuclear pore; cytosol; nucleus

Molecular Function: DNA binding; transferase activity, transferring acyl groups

Biological Process: mRNA transport; immune system process; protein import into nucleus; DNA replication