Human S-Phase Kinase Associated Protein 2 (SKP2) ELISA Kit | SKP2 elisa kit

Human S-Phase Kinase Associated Protein 2 (SKP2) ELISA Kit

Cat. Num. AAA2020011

• Gene Names: SKP2; p45; FBL1; FLB1; FBXL1
• Reactivity: Human
• Synonyms: S-Phase Kinase Associated Protein 2 (SKP2); Human S-Phase Kinase Associated Protein 2 (SKP2) ELISA Kit; FBL1; FBXL1; FLB1; p45; Cyclin-A/CDK2-associated protein p45; F-box/LRR-repeat protein 1; p45skp2; SKP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SKP2. No significant cross-reactivity or interference between SKP2 and analogues was observed.
• Sequence Length: 2

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SKP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SKP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SKP2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SKP2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SKP2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SKP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SKP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SKP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SKP2 elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 68161558
• NCBI GeneID: 6502
• Molecular Weight: 23,763 Da
• NCBI Official Full Name: S-phase kinase associated protein 2, partial
• NCBI Official Synonym Full Names: S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
• NCBI Official Symbol: SKP2
• NCBI Official Synonym Symbols: p45; FBL1; FLB1; FBXL1
• NCBI Protein Information: S-phase kinase-associated protein 2
• UniProt Protein Name: S-phase kinase-associated protein 2
• Protein Family: S-phase kinase-associated protein
• UniProt Gene Name: SKP2
• UniProt Synonym Gene Names: FBXL1
• UniProt Entry Name: SKP2_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011]

Uniprot Description

SKP2: an F-box protein of the SCF (SKP1-CUL1-F- box protein) E3 ubiquitin ligase system. The F-box component of the SCF complex is responsible for recognizing different substrates for ubiquitination. The SCF E3 complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Three alternatively-spliced isoforms have been described.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; SCF ubiquitin ligase complex; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity

Biological Process: regulation of apoptosis; cell proliferation; protein polyubiquitination; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of cell cycle; positive regulation of smooth muscle cell proliferation; positive regulation of estrogen receptor signaling pathway; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle