Canine Troponin T (TNT) ELISA Kit | TNT elisa kit

Canine Troponin T (TNT) ELISA Kit

Cat. Num. AAA2019960

• Reactivity: Canine
• Synonyms: Troponin T (TNT); Canine Troponin T (TNT) ELISA Kit; TNT elisa kit

• Reactivity: Canine
• Sequence Length: 11

• Samples: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Detection Range: 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL
• Sensitivity: 5.6pg/mL
• Preparation and Storage: For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T (TNT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T (TNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T (TNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin T (TNT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4261776
• Molecular Weight: 1,362 Da
• NCBI Official Full Name: troponin T, partial
• UniProt Protein Name: Troponin T protein
• UniProt Gene Name: troponin T
• UniProt Entry Name: V9H0A7_HUMAN

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]