Human Myozenin 2 (MYOZ2) ELISA Kit | MYOZ2 elisa kit

Human Myozenin 2 (MYOZ2) ELISA Kit

Cat. Num. AAA2019141

• Gene Names: MYOZ2; CS-1; CMH16; C4orf5
• Reactivity: Human
• Synonyms: Myozenin 2 (MYOZ2); Human Myozenin 2 (MYOZ2) ELISA Kit; CS-1; C4orf5; Calsarcin; Calcineurin Associated Sarcomeric Protein; FATZ-related protein 2; MYOZ2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myozenin 2 (MYOZ2).; No significant cross-reactivity or interference between Myozenin 2 (MYOZ2) and analogues was observed.
• Sequence Length: 264

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myozenin 2 (MYOZ2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myozenin 2 (MYOZ2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYOZ2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myozenin 2 (MYOZ2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myozenin 2 (MYOZ2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myozenin 2 (MYOZ2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myozenin 2 (MYOZ2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MYOZ2 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 119594068
• NCBI GeneID: 51778
• UniProt Accession #: Q9NPC6; O43415; Q9HB92
• Molecular Weight: 29,898 Da
• NCBI Official Full Name: myozenin 2
• NCBI Official Synonym Full Names: myozenin 2
• NCBI Official Symbol: MYOZ2
• NCBI Official Synonym Symbols: CS-1; CMH16; C4orf5
• NCBI Protein Information: myozenin-2
• UniProt Protein Name: Myozenin-2
• Protein Family: Myozenin
• UniProt Gene Name: MYOZ2
• UniProt Entry Name: MYOZ2_HUMAN

NCBI Description

The protein encoded by this gene belongs to a family of sarcomeric proteins that bind to calcineurin, a phosphatase involved in calcium-dependent signal transduction in diverse cell types. These family members tether calcineurin to alpha-actinin at the z-line of the sarcomere of cardiac and skeletal muscle cells, and thus they are important for calcineurin signaling. Mutations in this gene cause cardiomyopathy familial hypertrophic type 16, a hereditary heart disorder. [provided by RefSeq, Aug 2011]

Uniprot Description

MYOZ2: Myozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, gamma- filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the sarcomere. Plays an important role in the modulation of calcineurin signaling. May play a role in myofibrillogenesis. Defects in MYOZ2 are the cause of familial hypertrophic cardiomyopathy type 16 (CMH16). CMH16 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the myozenin family.

Protein type: Contractile; Cytoskeletal

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: sarcomere; Z disc; actin cytoskeleton

Molecular Function: protein binding; telethonin binding; actin binding; protein phosphatase 2B binding

Disease: Cardiomyopathy, Familial Hypertrophic, 16