Rat Macrophage Derived Chemokine (MDC) ELISA Kit | MDC elisa kit

Rat Macrophage Derived Chemokine (MDC) ELISA Kit

Cat. Num. AAA2018958

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Reactivity: Rat
• Synonyms: Macrophage Derived Chemokine (MDC); Rat Macrophage Derived Chemokine (MDC) ELISA Kit; CCL22; ABCD1; DC/B-CK; SCYA22; STCP1; Chemokine C-C-Motif Ligand 22; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22; Stimulated T-Cell Chemotactic Protein 1; MDC elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Macrophage Derived Chemokine (MDC).; No significant cross-reactivity or interference between Macrophage Derived Chemokine (MDC) and analogues was observed.
• Sequence Length: 93

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 23.44-1500pg/mL
• Sensitivity: < 8.47pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MDC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Derived Chemokine (MDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Derived Chemokine (MDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Derived Chemokine (MDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Derived Chemokine (MDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MDC elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 1931581
• NCBI GeneID: 6367
• UniProt Accession #: O00626; A0N0Q6; B2R4W2
• Molecular Weight: 10,625 Da
• NCBI Official Full Name: macrophage-derived chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22
• UniProt Protein Name: C-C motif chemokine 22
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22
• UniProt Entry Name: CCL22_HUMAN

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Chemokine

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: cell-cell signaling; response to virus; immune response; inflammatory response; chemotaxis; signal transduction