Heat Shock 70kDa Protein 9 (HSPA9) Recombinant Protein | HSPA9 recombinant protein

Recombinant Heat Shock 70kDa Protein 9 (HSPA9)

Cat. Num. AAA2018822

• Gene Names: HSPA9; CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heat Shock 70kDa Protein 9 (HSPA9); Recombinant Heat Shock 70kDa Protein 9 (HSPA9); HSPA9 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH 8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SCVAVM EGKQAKVLEN AEGARTTPSV VAFTADGERL VGMPAKRQAV TNPNNTFYAT KRLIGRRYDD PEVQKDIKNV PFKIVRASNG DAWVEAHGKL YSPSQIGAFV LMKMKETAEN YLGHTAKNAV ITVPAYFNDS QRQATKDAGQ ISGLNVLRVI NEPTAAALAY GLDKSEDKVI AVYDLGGGTF DISILEIQKGVFEVKSTNGD TFLGGEDFDQ ALLRHIVKEF KRETGVDLTK DNMALQRVRE A
• Sequence Length: 679

• Applicable Applications for HSPA9 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser65~Ala311 (Accession # P38646) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Mitochondrion. Nucleus, nucleolus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 24234688
• NCBI GeneID: 3313
• NCBI Accession #: NP_004125.3
• NCBI GenBank Nucleotide #: NM_004134.6
• UniProt Accession #: P38646; P30036; P31932; Q1HB43; Q53H23; Q6GU03; Q9BWB7; Q9UC56; B2RCM1
• Molecular Weight: 30.8kDa
• NCBI Official Full Name: stress-70 protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 70kDa protein 9 (mortalin)
• NCBI Official Symbol: HSPA9
• NCBI Official Synonym Symbols: CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
• NCBI Protein Information: stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein
• UniProt Protein Name: Stress-70 protein, mitochondrial
• Protein Family: Stress-70 protein
• UniProt Gene Name: HSPA9
• UniProt Synonym Gene Names: GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
• UniProt Entry Name: GRP75_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Uniprot Description

HSPA9B: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein; Chaperone; Nucleolus

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: focal adhesion; mitochondrion; cytoplasm; nucleolus

Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding

Biological Process: cellular protein metabolic process; protein folding; protein export from nucleus; protein targeting to mitochondrion; negative regulation of apoptosis