Protein Tyrosine Phosphatase Receptor Type M (PTPRM) Recombinant Protein | PTPRM recombinant protein

Recombinant Protein Tyrosine Phosphatase Receptor Type M (PTPRM)

Cat. Num. AAA2018544

• Gene Names: PTPRM; RPTPM; RPTPU; PTPRL1; hR-PTPu; R-PTP-MU
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Tyrosine Phosphatase Receptor Type M (PTPRM); Recombinant Protein Tyrosine Phosphatase Receptor Type M (PTPRM); PTPRM recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TPTL RVEDCSIALL PRNHEKNRCM DILPPDRCLP FLITIDGESS NYINAALMDS YKQPSAFIVT QHPLPNTVKD FWRLVLDYHC TSVVMLNDVD PAQLCPQYWP ENGVHRHGPI QVEFVSADLE EDIISRIFRI YNAARPQDGY RMVQQFQFLG WPMYRDTPVS KRSFLKLIRQ VDKWQEEYNGGEGRTVVHCL NGGGRSGTFC AIS
• Sequence Length: 1465

• Applicable Applications for PTPRM recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Thr1197~Ser1403 (Accession # P28827) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 157419152
• NCBI GeneID: 5797
• NCBI Accession #: NP_001098714.1
• NCBI GenBank Nucleotide #: NM_001105244.1
• UniProt Accession #: P28827; A7MBN1; D3DUH8; J3QL11
• Molecular Weight: 27.6kDa
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase mu isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, M
• NCBI Official Symbol: PTPRM
• NCBI Official Synonym Symbols: RPTPM; RPTPU; PTPRL1; hR-PTPu; R-PTP-MU
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase mu; protein tyrosine phosphatase mu; protein-tyrosine phosphatase mu; protein tyrosine phosphatase, receptor type, mu polypeptide
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase mu
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRM
• UniProt Synonym Gene Names: PTPRL1; Protein-tyrosine phosphatase mu; R-PTP-mu
• UniProt Entry Name: PTPRM_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion. Alternative splicing results in multiple transcripts encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PTPRM: a single-pass type I membrane protein involved in cell-cell adhesion through homophilic interactions. A protein-tyrosine phosphatase receptor of the class 2B subfamily. A negative regulator of proliferation and invasion of breast cancer cells. The PTPRM gene is frequently hypermethylated in sporadic cancers. Modulates p120 catenin phosphorylation, promoting adipogenic differentiation. Two isoforms of the human protein have been reported.

Protein type: Phosphatase; EC 3.1.3.48; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18p11.2

Cellular Component: cell-cell adherens junction; lamellipodium; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasm; intercellular junction

Molecular Function: identical protein binding; protein binding; cadherin binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: response to drug; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; homophilic cell adhesion; signal transduction; positive regulation of vasodilation; protein amino acid dephosphorylation; neurite development; retinal ganglion cell axon guidance