Protease, Serine 1 (PRSS1) Recombinant Protein | PRSS1 recombinant protein

Recombinant Protease, Serine 1 (PRSS1)

Cat. Num. AAA2018507

• Gene Names: PRSS1; TRP1; TRY1; TRY4; TRYP1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protease; Serine 1 (PRSS1); Recombinant Protease; PRSS1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SLINEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPQYD RKTLNNDIML IKLSSRAVIN ARVSTISLPT APPATGTKCL ISGWGNTASS GADYPDELQC LDAPVLSQAK CEASYPGKIT SNMFCVGFLE GGKDSCQGDS GGPVVCNGQL QGVVSWGDGC AQKNKPGV
• Sequence Length: 247

• Applicable Applications for PRSS1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser51~Val228 (Accession # P07477) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4506145
• NCBI GeneID: 5644
• NCBI Accession #: NP_002760.1
• NCBI GenBank Nucleotide #: NM_002769.4
• UniProt Accession #: P07477; Q5NV57; Q7M4N3; Q7M4N4; Q92955; Q9HAN4; Q9HAN5; Q9HAN6; Q9HAN7; A1A509; A6NJ71; B2R5I5
• Molecular Weight: 22.8kDa
• NCBI Official Full Name: trypsin-1 preproprotein
• NCBI Official Synonym Full Names: protease, serine, 1 (trypsin 1)
• NCBI Official Symbol: PRSS1
• NCBI Official Synonym Symbols: TRP1; TRY1; TRY4; TRYP1
• NCBI Protein Information: trypsin-1; beta-trypsin; trypsinogen 1; trypsinogen A; digestive zymogen; cationic trypsinogen; nonfunctional trypsin 1
• UniProt Protein Name: Trypsin-1
• Protein Family: Anionic trypsin
• UniProt Gene Name: PRSS1
• UniProt Synonym Gene Names: TRP1; TRY1; TRYP1
• UniProt Entry Name: TRY1_HUMAN

NCBI Description

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]

Uniprot Description

trypsin 1: Has activity against the synthetic substrates Boc-Phe- Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val- Pro-Arg-Mec. The single-chain form is more active than the two- chain form against all of these substrates. Defects in PRSS1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.21.4

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase activity; metal ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; vitamin metabolic process; cobalamin metabolic process; digestion; proteolysis; water-soluble vitamin metabolic process

Disease: Trypsinogen Deficiency; Pancreatitis, Hereditary