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SDS-Page

UDP Glycosyltransferase 8 (UGT8) Recombinant Protein | UGT8 recombinant protein

Recombinant UDP Glycosyltransferase 8 (UGT8)

Gene Names
UGT8; CGT; UGT4
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
UDP Glycosyltransferase 8 (UGT8); Recombinant UDP Glycosyltransferase 8 (UGT8); UGT8 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S- GVY LISRLGVSFL VLPKYERIMQ KYNLLPEKSM YDLVHGSSLW MLCTDVALEF PRPTLPNVVY VGGILTKPAS PLPEDLQRWV NGANEHGFVL VSFGAGVKYL SEDIANKLAG ALGRLPQKVI WRFSGPKPKN LGNNTKLIEW LPQNDLLGHS KIKAFLSHGG LNSIFETIYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE WKTVTEKELY EALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTIYWIDY IIRHNGAHHL RAAVHQISFC QYFLLDIAFV LLLGAALLYF LLSWVTKFIY RKIKSLWSRN KHSTVNGHYH NGILNGKYKR NGHIKHEKKV K
Sequence Length
541
Applicable Applications for UGT8 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Gly198~Lys541 (Accession # Q16880) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40.5kDa
NCBI Official Full Name
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
NCBI Official Synonym Full Names
UDP glycosyltransferase 8
NCBI Official Symbol
UGT8
NCBI Official Synonym Symbols
CGT; UGT4
NCBI Protein Information
2-hydroxyacylsphingosine 1-beta-galactosyltransferase; cerebroside synthase; ceramide UDP-galactosyltransferase; uridine diphosphate glycosyltransferase 8; UDP-galactose ceramide galactosyltransferase; UDP-galactose-ceramide galactosyltransferase
UniProt Protein Name
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
UniProt Gene Name
UGT8
UniProt Synonym Gene Names
CGT; UGT4
UniProt Entry Name
CGT_HUMAN

NCBI Description

The protein encoded by this gene belongs to the UDP-glycosyltransferase family. It catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central and peripheral nervous systems. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

UGT8: Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Belongs to the UDP-glycosyltransferase family.

Protein type: Lipid Metabolism - sphingolipid; Transferase; Membrane protein, integral; EC 2.4.1.45

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: intracellular membrane-bound organelle; integral to membrane

Molecular Function: UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity; glucuronosyltransferase activity; 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity

Biological Process: central nervous system development; flavonoid biosynthetic process; galactosylceramide biosynthetic process; axon cargo transport; cytoskeleton organization and biogenesis; neurite morphogenesis; paranodal junction assembly; cellular response to hormone stimulus; peripheral nervous system development

Research Articles on UGT8

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Product Notes

The UGT8 ugt8 (Catalog #AAA2018221) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UDP Glycosyltransferase 8 (UGT8) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the UGT8 ugt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG S- GVY LISRLGVSFL VLPKYERIMQ KYNLLPEKSM YDLVHGSSLW MLCTDVALEF PRPTLPNVVY VGGILTKPAS PLPEDLQRWV NGANEHGFVL VSFGAGVKYL SEDIANKLAG ALGRLPQKVI WRFSGPKPKN LGNNTKLIEW LPQNDLLGHS KIKAFLSHGG LNSIFETIYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE WKTVTEKELY EALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTIYWIDY IIRHNGAHHL RAAVHQISFC QYFLLDIAFV LLLGAALLYF LLSWVTKFIY RKIKSLWSRN KHSTVNGHYH NGILNGKYKR NGHIKHEKKV K. It is sometimes possible for the material contained within the vial of "UDP Glycosyltransferase 8 (UGT8), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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