Rabbit Interleukin 7 Receptor (IL7R) Polyclonal Antibody | anti-IL7R antibody

Polyclonal Antibody to Interleukin 7 Receptor (IL7R)

Cat. Num. AAA2018052

• Gene Names: IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
• Reactivity: Homo sapiens (Human)
• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Interleukin 7 Receptor (IL7R); Polyclonal Antibody; Polyclonal Antibody to Interleukin 7 Receptor (IL7R); anti-IL7R antibody

• Host: Rabbit
• Reactivity: Homo sapiens (Human)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IL7R antibody: Western Blot (WB); Immunocytochemistry (ICC); Immunohistochemistry – Paraffin (IHC-P); Immunohistochemistry – Frozen (IHC-Fr); ELISA
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Immunogen: Recombinant IL7R (Lys173~Ala260 (Accession # P16871)) expressed in E.coli (MBS2018753).
• Preparation and Storage: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Western Blot (WB)

(Western Blot:Sample: Recombinant IL7R, Human.)

Western Blot (WB)

(Western Blot:Sample: Lane1: Human Lung Tissue;Lane2: Human K562 Cells;Lane3: Human Jurkat Cells.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Stomach Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Skin Cancer Tissue)

Knockout Validation

(Knockout Validation:Lane 1: Wild-type K562 cell lysate;Lane 2: IL7R knockout K562 cell lysate;Predicted MW: 51,34,29kDa ;Observed MW: 60kDa;Primary Ab: 2ug/ml Rabbit Anti-Human IL7R Ab;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Product Categories/Family for anti-IL7R antibody

Knock-Out Validation Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 28610151
• NCBI GeneID: 3575
• NCBI Accession #: NP_002176.2
• NCBI GenBank Nucleotide #: NM_002185.4
• NCBI Official Full Name: interleukin-7 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 7 receptor
• NCBI Official Symbol: IL7R
• NCBI Official Synonym Symbols: ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
• NCBI Protein Information: interleukin-7 receptor subunit alpha
• UniProt Protein Name: Interleukin-7 receptor subunit alpha
• Protein Family: Interleukin-7 receptor
• UniProt Gene Name: IL7R
• UniProt Synonym Gene Names: IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA

NCBI Description

The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015]

Uniprot Description

IL7R: Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell- positive/NK-cell-positive (T(-)B(+)NK(+) SCID). A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS. Belongs to the type I cytokine receptor family. Type 4 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5p13.2

Cellular Component: external side of plasma membrane; extracellular region; integral component of membrane; plasma membrane

Molecular Function: antigen binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-7 receptor activity; protein binding

Biological Process: B cell proliferation; cell growth; cell morphogenesis; cell surface receptor signaling pathway; homeostasis of number of cells; immune response; immunoglobulin production; interleukin-7-mediated signaling pathway; lymph node development; negative regulation of T cell mediated cytotoxicity; positive regulation of cell proliferation; positive regulation of gene expression; positive regulation of STAT cascade; positive regulation of T cell differentiation in thymus; regulation of cell size; regulation of DNA recombination; signal transduction; T cell differentiation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive