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Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse FUT4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Fucosyltransferase 4 (FUT4) Polyclonal Antibody | anti-FUT4 antibody
Polyclonal Antibody to Fucosyltransferase 4 (FUT4)
Gene Names
Fut4; FAL; LeX; CD15; Ssea1; SSEA-1; FucT-IV; AI451562
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Fucosyltransferase 4 (FUT4); Polyclonal Antibody; Polyclonal Antibody to Fucosyltransferase 4 (FUT4); anti-FUT4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FUT4. It has been selected for its ability to recognize FUT4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VKELHDW PPPWGARERT DKALVLRVFD DQEGAVTLTG KALETVGSRP PGQRWVWMNF ESPSHTPGLR GLAKDLFNWT LSYRTDSDVF VPYGFLYSRS DPTEQPSGLG PQLARKRGLV AWVVSNWNEH QARVRYYHQL SRHVSVDVFG RTGPGRPVPA IGLLHTVARY KFYLAFENSR HVDYITEKLW RNAFLAGAVP VVLGPDRANY ERFVPRGAFI HVDDFPNAAS LAAYLLFLDR
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VKELHDW PPPWGARERT DKALVLRVFD DQEGAVTLTG KALETVGSRP PGQRWVWMNF ESPSHTPGLR GLAKDLFNWT LSYRTDSDVF VPYGFLYSRS DPTEQPSGLG PQLARKRGLV AWVVSNWNEH QARVRYYHQL SRHVSVDVFG RTGPGRPVPA IGLLHTVARY KFYLAFENSR HVDYITEKLW RNAFLAGAVP VVLGPDRANY ERFVPRGAFI HVDDFPNAAS LAAYLLFLDR
Sequence Length
349
Applicable Applications for anti-FUT4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant FUT4 (Val144~Phe389) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051054
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse FUT4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse FUT4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,698 Da
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 4
NCBI Official Synonym Full Names
fucosyltransferase 4
NCBI Official Symbol
Fut4
NCBI Official Synonym Symbols
FAL; LeX; CD15; Ssea1; SSEA-1; FucT-IV; AI451562
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 4
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 4
Protein Family
UniProt Gene Name
Fut4
UniProt Synonym Gene Names
Elft; Fuc-TIV; FucT-IV
Uniprot Description
FUT4: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Belongs to the glycosyltransferase 10 family.
Protein type: EC 2.4.1.-; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; Transferase
Chromosomal Location of Human Ortholog: 9 A2|9 4.3 cM
Cellular Component: cell surface; Golgi apparatus; Golgi membrane; integral component of membrane; membrane
Molecular Function: alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity; transferase activity; transferase activity, transferring glycosyl groups
Biological Process: protein glycosylation
Research Articles on FUT4
Similar Products
Product Notes
The FUT4 fut4 (Catalog #AAA2013565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Fucosyltransferase 4 (FUT4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Fucosyltransferase 4 (FUT4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the FUT4 fut4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VKELHDW PPPWGARERT DKALVLRVFD DQEGAVTLTG KALETVGSRP PGQRWVWMNF ESPSHTPGLR GLAKDLFNWT LSYRTDSDVF VPYGFLYSRS DPTEQPSGLG PQLARKRGLV AWVVSNWNEH QARVRYYHQL SRHVSVDVFG RTGPGRPVPA IGLLHTVARY KFYLAFENSR HVDYITEKLW RNAFLAGAVP VVLGPDRANY ERFVPRGAFI HVDDFPNAAS LAAYLLFLDR. It is sometimes possible for the material contained within the vial of "Fucosyltransferase 4 (FUT4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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