LILRB2/CD85d/ILT4 recombinant protein

Recombinant human LILRB2/CD85d/ILT4 protein

Cat. Num. AAA201355

• Gene Names: LILRB2; ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: LILRB2/CD85d/ILT4; Recombinant human LILRB2/CD85d/ILT4 protein; Leukocyte Immunoglobulin-like Receptor; CD85D; CD85d; CD85 antigen-like family member D; CD85d antigen; ILT-4; ILT4; ILT4CD85d; Ig-like transcript 4; Immunoglobulin-like transcript 4; LILRB2; LIR2; LIR-2; LIR2CD85D; Leukocyte immunoglobulin-like receptor 2; MIR10; MIR-10; MIR10LILRA6; Monocyte/macrophage immunoglobulin-like receptor 10; leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform 1; LILRB2/CD85d/ILT4 recombinant protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 20% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 24-461aa
• Sequence: GTIPKPTLWA EPDSVITQGS PVTLSCQGSL EAQEYRLYRE KKSASWITRI RPELVKNGQF HIPSITWEHT GRYGCQYYSR ARWSELSDPL VLVMTGAYPK PTLSAQPSPV VTSGGRVTLQ CESQVAFGGF ILCKEGEDEH PQCLNSQPHA RGSSRAIFSV GPVSPNRRWS HRCYGYDLNS PYVWSSPSDL LELLVPGVSK KPSLSVQPGP VMAPGESLTL QCVSDVGYDR FVLYKEGERD LRQLPGRQPQ AGLSQANFTL GPVSRSYGGQ YRCYGAHNLS SECSAPSDPL DILITGQIRG TPFISVQPGP TVASGENVTL LCQSWRQFHT FLLTKAGAAD APLRLRSIHE YPKYQAEFPM SPVTSAHAGT YRCYGSLNSD PYLLSHPSEP LELVVSGPSM GSSPPPTGPI STPAGPEDQP LTPTGSDPQS GLGRHLGV

• Applicable Applications for LILRB2/CD85d/ILT4 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Gly24
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for LILRB2/CD85d/ILT4 recombinant protein

LILRB2, also known as CD85d, LIR-2, and MIR10, is a member of the leukocyte immunoglobulin-like receptor (LIR) family. It is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. Among them it competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1. And it binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules. This protein is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Recombinant human LILRB2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for LILRB2/CD85d/ILT4 recombinant protein

Immunology; Proteins

References

Fanger, N.A. et al. (1998) Eur. J. Immunol. 28:3423. Baudhuin, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:17957-17962. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856-8861. Borges, L. et al. (1997) J. Immunol. 159:5192-5196.

NCBI and Uniprot Product Information

• NCBI GI #: 125988400
• NCBI GeneID: 10288
• NCBI Accession #: NP_005865
• NCBI GenBank Nucleotide #: NM_005874
• UniProt Accession #: Q8N423
• Molecular Weight: 65kDa
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 2 isoform 1
• NCBI Official Synonym Full Names: leukocyte immunoglobulin like receptor B2
• NCBI Official Symbol: LILRB2
• NCBI Official Synonym Symbols: ILT4; LIR2; CD85D; ILT-4; LIR-2; MIR10; MIR-10
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 2
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 2
• UniProt Gene Name: LILRB2
• UniProt Synonym Gene Names: ILT4; LIR2; MIR10; LIR-2; Leukocyte immunoglobulin-like receptor 2; ILT-4; MIR-10
• UniProt Entry Name: LIRB2_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]