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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Fucosidase Alpha L1, Tissue (FUCa1) Polyclonal Antibody | anti-FUCa1 antibody

Polyclonal Antibody to Fucosidase Alpha L1, Tissue (FUCa1)

Gene Names
Fuca1; Fuca
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Fucosidase Alpha L1; Tissue (FUCa1); Polyclonal Antibody; Polyclonal Antibody to Fucosidase Alpha L1; anti-FUCa1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FUCa1. It has been selected for its ability to recognize FUCa1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K RNIRYGLYHS LFEWFHPLYL LDKKNGLKTQ HFVSTKTMPE LYDLVNRYKP DLIWSDGEWE CPDSYWNSTE FLAWLYNESP VKDQVVVNDR WGQNCSCRHG GYYNCEDKYR PHSLPDHKWE MCTSVDKASW GYRRDMSMST IVDENEIIEE LVQTISLGGN YLLNIGPNKD GVIVPIFQER LLAVGKWLQI NGEAIYASKP WRVQSERNKT VVWYTTKDSA VYATFLHWPE DGVVNLQSPK MTSATKITML
Sequence Length
451
Applicable Applications for anti-FUCa1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant FUCa1 (Lys170~Leu420) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047780
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,487 Da
NCBI Official Full Name
tissue alpha-L-fucosidase
NCBI Official Synonym Full Names
alpha-L-fucosidase 1
NCBI Official Symbol
Fuca1
NCBI Official Synonym Symbols
Fuca
NCBI Protein Information
tissue alpha-L-fucosidase
UniProt Protein Name
Tissue alpha-L-fucosidase
Protein Family
UniProt Gene Name
Fuca1
UniProt Synonym Gene Names
Fuca; Alpha-L-fucosidase 1

NCBI Description

catalyzes the hydrolysis of an alpha-L-fucoside to an L-fucose [RGD, Feb 2006]

Uniprot Description

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

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Product Notes

The FUCa1 fuca1 (Catalog #AAA2013320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Fucosidase Alpha L1, Tissue (FUCa1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fucosidase Alpha L1, Tissue (FUCa1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the FUCa1 fuca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K RNIRYGLYHS LFEWFHPLYL LDKKNGLKTQ HFVSTKTMPE LYDLVNRYKP DLIWSDGEWE CPDSYWNSTE FLAWLYNESP VKDQVVVNDR WGQNCSCRHG GYYNCEDKYR PHSLPDHKWE MCTSVDKASW GYRRDMSMST IVDENEIIEE LVQTISLGGN YLLNIGPNKD GVIVPIFQER LLAVGKWLQI NGEAIYASKP WRVQSERNKT VVWYTTKDSA VYATFLHWPE DGVVNLQSPK MTSATKITML. It is sometimes possible for the material contained within the vial of "Fucosidase Alpha L1, Tissue (FUCa1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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