SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
)
NCAM-1/CD56 recombinant protein
Recombinant human NCAM-1/CD56 protein
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
NCAM-1/CD56; Recombinant human NCAM-1/CD56 protein; Neural cell adhesion molecule 1; Neural cell adhesion molecule 1 isoform3; N-CAM-1; NCAM-1; NCAM; NCAM1; CD56 antigen; CD56; MSK39; NCAM-1/CD56 recombinant protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
20-603aa
Sequence
Applicable Applications for NCAM-1/CD56 recombinant protein
SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Human
Tag
His-Tag
Predicted N-term
Ala
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
)
Related Product Information for NCAM-1/CD56 recombinant protein
NCAM-1, also known as CD56, is a member of the immunoglobulin superfamily. It specifically binds to neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. This protein is preferentially expressed in NK cells and a subset of T lymphocytes that mediate MHC-unrestricted cell-mediated cytotoxicity. High expression of NCAM-1/CD56 differentiates NK cells as having an activated phenotype. CD56(+high) NK cells mediate heightened effector functions in response to IL-12. During hematopoiesis, NCAM-1/CD56 is the prototypic marker of NK cells, also present on subset of CD4+ T cells and CD8+ T cells. In cell adhesion, it contributes to cell-cell adhesion or cell-matrix adhesion during embryonic development. In anatomic pathology, pathologists make use of it immunohistochemistry to recognize certain tumors. Recombinant human NCAM-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for NCAM-1/CD56 recombinant protein
References
Van Acker HH, et al. (2017). Frontiers in Immunology. 8:892. Sanchez-Heras, E. et al. (2006) J Biol Chem. 281:35208-35216.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
65.7kDa(593aa)
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Gene Name
NCAM1
UniProt Synonym Gene Names
NCAM; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_HUMAN
Uniprot Description
NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q23.1
Cellular Component: Golgi membrane; cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: identical protein binding
Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion
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Product Notes
The NCAM-1/CD56 ncam1 (Catalog #AAA201304) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 20-603aa. AAA Biotech's NCAM-1/CD56 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NCAM-1/CD56 ncam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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