Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human ACAT1 Monoclonal Antibody | anti-ACAT1 antibody
ACAT1
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Hep3B cell lysate Lane 4.: 293T cell lysate Lane 5.: A431 cell lysate )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Hep3B cell lysate Lane 4.: 293T cell lysate Lane 5.: A431 cell lysate )
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
Uniprot Description
ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.
Protein type: Mitochondrial; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - lysine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - butanoate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Acetyltransferase; Amino Acid Metabolism - tryptophan
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane
Molecular Function: protein homodimerization activity; enzyme binding; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding
Biological Process: response to starvation; ketone body catabolic process; response to hormone stimulus; ketone body biosynthetic process; branched chain family amino acid catabolic process; cellular lipid metabolic process; brain development; ketone body metabolic process; liver development; response to organic cyclic substance; protein homooligomerization
Disease: Alpha-methylacetoacetic Aciduria
Research Articles on ACAT1
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Product Notes
The ACAT1 acat1 (Catalog #AAA201225) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACAT1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACAT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Researchers should empirically determine the suitability of the ACAT1 acat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACAT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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