Collagen Type VIII Alpha 1 (COL8a1) Recombinant Protein | COL8a1 recombinant protein

Recombinant Collagen Type VIII Alpha 1 (COL8a1)

Cat. Num. AAA2011566

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Collagen Type VIII Alpha 1 (COL8a1); Recombinant Collagen Type VIII Alpha 1 (COL8a1); COL8a1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-LGIDGVKTPH AYAAKKGKNG GPAYEMPAFT AELTAPFPPV GAPIKFDRLL YNGRQNYNPQ TGIFTCEVPG VYYFAYHVHC KGGNVWVALF KNNEPVMYTY DEYKKGFLDQ ASGSAVLLLR PGDRVFLQMP SEQAAGLYAG QYVHSSFSGY LLYPM
• Sequence Length: 744

• Applicable Applications for COL8a1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu590~Met744 (Accession # P14282) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 130487849
• NCBI GeneID: 100009383
• NCBI Accession #: NP_001076135.1
• NCBI GenBank Nucleotide #: NM_001082666.1
• UniProt Accession #: P14282
• Molecular Weight: 18.6kDa
• NCBI Official Full Name: collagen alpha-1(VIII) chain
• NCBI Official Symbol: COL8A1
• NCBI Protein Information: collagen alpha-1(VIII) chain; collagen alpha-1(VIII) chain; endothelial collagen; alpha 1 type VIII collagen
• UniProt Protein Name: Collagen alpha-1(VIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL8A1
• UniProt Entry Name: CO8A1_RABIT

Uniprot Description

Function: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis

By similarity.Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis

By similarity.

Subunit structure: Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers

By similarity.

Subcellular location: Secreted › extracellular space › extracellular matrix › basement membrane.

Post-translational modification: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin

By similarity.

Miscellaneous: 4 consecutive G-P-P tripeptides are present at the C-terminus of the triple-helical region. These may provide high thermal stability of this region.

Sequence similarities: Contains 1 C1q domain.