CD72 recombinant protein

Recombinant CD72

Cat. Num. AAA201134

• Gene Names: CD72; LYB2; CD72b
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: CD72; Recombinant CD72; CD72b; LYB2; B-cell differentiation antigen CD72; Lyb-2; CD_antigen: CD72; CD72 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 50mM Tris-HCl (pH 6.8) Containing 2mM DTT, 0.2M NaCl, 50% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 117-359aa
• Sequence: ADP RYLQVSQ QLQQTNRVLE VTNSSLRQQL RLKITQLGQS AEDLQGSRRE LAQSQEALQV EQRAHQAAEG QLQACQADRQ KTKETLQSEE QQRRALEQKL SNMENRLKPF FTCGSADTCC PSGWIMHQKS CFYISLTSKN WQESQKQCET LSSKLATFSE IYPQSHSYYF LNSLLPNGGS GNSYWTGLSS NKDWKLTDDT QRTRTYAQSS KCNKVHKTWS WWTLESESCR SSLPYICEMT AFRFPD LEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
• Sequence Length: 359

• Applicable Applications for CD72 recombinant protein: SDS-PAGE
• Species: Human
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: hIgG-His-Tag
• Predicted N-Terminal: Ala
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for CD72 recombinant protein

CD72, also known as B-cell differentiation antigen CD72, is a type II transmembrane glycoprotein carrying a C-type lectin domain. It plays a role in immune system regulation of animals and expressed on B lineage cells, NK cells, monocytes, dendritic cells, and mast cells as a homodimer. Also, this protein associates with CD79A in the B cell antigen receptor (BCR) complex following antigen stimulation and dampens BCR signaling through interactions with the phosphatase SHP1. It binding to Semaphorin 4D induces cytokine production by monocytes and dendritic cells, inhibits SCF R/ckit induced mast cell proliferation and activation, and inhibits the cytolytic activity of NK cells. Moreover, BCR signaling enhances phosphorylation of CD72 and its recruitment of SHP-1. Recombinant human CD72, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD72 recombinant protein

Immunology

References

Vadasz Z., et al, (2014) Semin. Arthritis Rheum. 43:767-771. Adachi T., et al, (1998) J. Immunol. 160:4662-4665.

NCBI and Uniprot Product Information

• NCBI GI #: 4502683
• NCBI GeneID: 971
• NCBI Accession #: NP_001773.1
• NCBI GenBank Nucleotide #: NP_001773.1
• UniProt Accession #: P21854
• Molecular Weight: 55.3kDa (485aa); 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: B-cell differentiation antigen CD72
• NCBI Official Synonym Full Names: CD72 molecule
• NCBI Official Symbol: CD72
• NCBI Official Synonym Symbols: LYB2; CD72b
• NCBI Protein Information: B-cell differentiation antigen CD72
• UniProt Protein Name: B-cell differentiation antigen CD72
• Protein Family: B-cell differentiation antigen
• UniProt Gene Name: CD72
• UniProt Entry Name: CD72_HUMAN

Uniprot Description

CD72: Plays a role in B-cell proliferation and differentiation.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding; receptor binding

Biological Process: axon guidance; signal transduction; cell adhesion