GFRA3 recombinant protein

GFRA3, 32-374aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206635

• Gene Names: GFRA3; GDNFR3
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: GFRA3; 32-374aa; Human; hIgG-His-tag; Baculovirus; GDNF family receptor alpha-3; GDNFR3; GFRalpha3; GFRA3 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPDPLPTES RLMNSCLQAR RKCQADPTCS AAYHHLDSCT SSISTPLPSE EPSVPADCLE AAQQLRNSSL IGCMCHRRMK NQVACLDIYW TVHRARSLGN YELDVSPYED TVTSKPWKMN LSKLNMLKPD SDLCLKFAML CTLNDKCDRL RKAYGEACSG PHCQRHVCLR QLLTFFEKAA EPHAQGLLLC PCAPNDRGCG ERRRNTIAPN CALPPVAPNC LELRRLCFSD PLCRSRLVDF QTHCHPMDIL GTCATEQSRC LRAYLGLIGT AMTPNFVSNV NTSVALSCTC RGSGNLQEEC EMLEGFFSHN PCLTEAIAAK MRFHSQLFSQ DWPHPTFAVM AHQNENLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
• Sequence Length: 400

• Applicable Applications for GFRA3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GFRA3 recombinant protein

GFRA3, also known as GDNF family receptor alpha-3, is a plasma membrane glycoprotein. This protein is one of the four GDNF receptors, all of which are glycosylphosphoinositol (GPI)-linked, contain three conserved cysteine repeats, and involved survival of neurons. Also, it promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. Recombinant human GFRA3, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for GFRA3 recombinant protein

Neuroscience; Development

References

Wu ZS., et al, (2013) BMC Cancer 13:34.; Baloh RH., et al, (1998) Neuron 21:1291-1302.

NCBI and Uniprot Product Information

• NCBI GI #: 22035694
• NCBI GeneID: 2676
• NCBI Accession #: NP_001487
• NCBI GenBank Nucleotide #: NM_001496.3
• UniProt Accession #: O60609; Q6UW20; Q8IUZ2; B2RA36; B4DMY9
• Molecular Weight: 65.5kDa (585aa) 70-100kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: GDNF family receptor alpha-3 preproprotein
• NCBI Official Synonym Full Names: GDNF family receptor alpha 3
• NCBI Official Symbol: GFRA3
• NCBI Official Synonym Symbols: GDNFR3
• NCBI Protein Information: GDNF family receptor alpha-3
• UniProt Protein Name: GDNF family receptor alpha-3
• Protein Family: GDNF family receptor
• UniProt Gene Name: GFRA3
• UniProt Synonym Gene Names: GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3

NCBI Description

The protein encoded by this gene is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.