Carboxypeptidase N1 (CPN1) Recombinant Protein | CPN1 recombinant protein

Recombinant Carboxypeptidase N1 (CPN1)

Cat. Num. AAA2011267

• Gene Names: Cpn1; CPN; 0610011F20Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Carboxypeptidase N1 (CPN1); Recombinant Carboxypeptidase N1 (CPN1); CPN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-VANYPYDKSL EHRFRGPHRT SNSPTPDDEL FQTLAKVYSY AHGWMHQGWN CGDYFPDGIT NGASWYSLSK GMQDFNYLHT NCFEITLELS CDKFPRQEEL QREWLGNREA LIQFLEQVHQ GIKGMVLDEN SNNLTGAVIS VTGINHDVTS GEHGDYFRLL LPGTYSVTAK APGYDPKTVT VTVGPAGPTV VDFQLKRSSS QVYPVQRAPG RGQGGRAKQP RTSRKKDPA
• Sequence Length: 457

• Applicable Applications for CPN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val221~Ala449 (Accession # Q9JJN5) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted, extracellular space
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13507644
• NCBI GeneID: 93721
• NCBI Accession #: NP_109628.1
• NCBI GenBank Nucleotide #: NM_030703.2
• UniProt Accession #: Q9JJN5; Q91WM9
• Molecular Weight: 57.6kDa
• NCBI Official Full Name: carboxypeptidase N catalytic chain
• NCBI Official Synonym Full Names: carboxypeptidase N, polypeptide 1
• NCBI Official Symbol: Cpn1
• NCBI Official Synonym Symbols: CPN; 0610011F20Rik
• NCBI Protein Information: carboxypeptidase N catalytic chain; kininase I; anaphylatoxin inactivator; carboxypeptidase N small subunit; carboxypeptidase N, polypeptide 1, 50kD
• UniProt Protein Name: Carboxypeptidase N catalytic chain
• Protein Family: Carboxypeptidase
• UniProt Gene Name: Cpn1
• UniProt Synonym Gene Names: CPN
• UniProt Entry Name: CBPN_MOUSE

Uniprot Description

CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.

Protein type: Secreted, signal peptide; Protease; EC 3.4.17.3; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; carboxypeptidase activity; hydrolase activity; zinc ion binding; metallopeptidase activity; metal ion binding; metallocarboxypeptidase activity

Biological Process: response to glucocorticoid stimulus; proteolysis