Caspase 7 (CASP7) Recombinant Protein | CASP7 recombinant protein

Recombinant Caspase 7 (CASP7)

Cat. Num. AAA2011088

• Gene Names: CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Caspase 7 (CASP7); Recombinant Caspase 7 (CASP7); CASP7 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ANPR YKIPVEADFL FAYSTVPGYY SWRSPGRGSW FVQALCSILE EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP HFHEKKQIPC VVSMLTKELY FSQ
• Sequence Length: 303

• Applicable Applications for CASP7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala207~Gln303 (Accession # P55210) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4502581
• NCBI GeneID: 840
• NCBI Accession #: NP_001218.1
• NCBI GenBank Nucleotide #: NM_001227.4
• UniProt Accession #: P55210; Q13364; Q53YD5; Q5SVL0; Q5SVL3; Q96BA0; B4DQU7; B5BU45; D3DRB8
• Molecular Weight: 15.2kDa
• NCBI Official Full Name: caspase-7 isoform alpha
• NCBI Official Synonym Full Names: caspase 7, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP7
• NCBI Official Synonym Symbols: MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• NCBI Protein Information: caspase-7; apoptotic protease MCH-3; ICE-like apoptotic protease 3; caspase 7, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-7
• Protein Family: Caspase
• UniProt Gene Name: CASP7
• UniProt Synonym Gene Names: MCH3; CASP-7; ICE-LAP3
• UniProt Entry Name: CASP7_HUMAN

Uniprot Description

CASP7: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Endoplasmic reticulum; Protease; Apoptosis; EC 3.4.22.60

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: apoptosis; caspase activation via cytochrome c; proteolysis; cell structure disassembly during apoptosis