Laminin Beta 3 (LAMb3) Recombinant Protein | LAMb3 recombinant protein

Recombinant Laminin Beta 3 (LAMb3)

Cat. Num. AAA2011064

• Gene Names: LAMB3; LAM5; LAMNB1; BM600-125KDA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Laminin Beta 3 (LAMb3); Recombinant Laminin Beta 3 (LAMb3); LAMb3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-GKNCERCQL HYFRNRRPGA SIQETCISCE CDPDGAVPGA PCDPVTGQCV CKEHVQGERC DLCKPGFTGL TYANPQGCHR CDCNILGSRR DMPCDEESGR CLCLPNVVGP KCDQCAPYHW KLASGQGCEP CACDPHNSLS PQCNQFTGQC PCREGFGGLM CSAAAIRQCP DRTYGDVATG CRACDCDFRG TEGPGCDKAS GRCLCRPGLT GPRCDQCQRG YCNRYPVCVA CHPCFQT
• Sequence Length: 1172

• Applicable Applications for LAMb3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Gly352~Thr587 (Accession # Q13751) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 62868215
• NCBI GeneID: 3914
• NCBI Accession #: NP_000219.2
• NCBI GenBank Nucleotide #: NM_000228.2
• UniProt Accession #: Q13751; O14947; Q14733; Q9UJK4; Q9UJL1; D3DT88
• Molecular Weight: 57.9kDa
• NCBI Official Full Name: laminin subunit beta-3
• NCBI Official Synonym Full Names: laminin, beta 3
• NCBI Official Symbol: LAMB3
• NCBI Official Synonym Symbols: LAM5; LAMNB1; BM600-125KDA
• NCBI Protein Information: laminin subunit beta-3; nicein-125kDa; kalinin-140kDa; kalinin B1 chain; laminin B1k chain; laminin S B3 chain; nicein subunit beta; kalinin subunit beta; epiligrin subunit bata; laminin-5 subunit beta; laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD))
• UniProt Protein Name: Laminin subunit beta-3
• Protein Family: Laminin
• UniProt Gene Name: LAMB3
• UniProt Synonym Gene Names: LAMNB1
• UniProt Entry Name: LAMB3_HUMAN

NCBI Description

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMB3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). GABEB is a non- lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: laminin-5 complex; extracellular region

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; hemidesmosome assembly; epidermis development; brown fat cell differentiation; cell adhesion

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Epidermolysis Bullosa, Junctional, Herlitz Type; Amelogenesis Imperfecta, Type Ia