Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Recombinant Protein | TAFI recombinant protein

Recombinant Thrombin Activatable Fibrinolysis Inhibitor (TAFI)

Cat. Num. AAA2010891

• Gene Names: Cpb2; CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Thrombin Activatable Fibrinolysis Inhibitor (TAFI); Recombinant Thrombin Activatable Fibrinolysis Inhibitor (TAFI); TAFI recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-HS YSQQILFPYS YNRSKSKDHE ELSLVASEAV RAIESINKNT RYTHGSGSES LYLAPGGSDD WIYD
• Sequence Length: 422

• Applicable Applications for TAFI recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: His309~Asp374 (Accession # Q9JHH6) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31982712
• NCBI GeneID: 56373
• NCBI Accession #: NP_062749.2
• NCBI GenBank Nucleotide #: NM_019775.3
• UniProt Accession #: Q9JHH6; Q5EBI3; Q9QZF0
• Molecular Weight: 13.1kDa
• NCBI Official Full Name: carboxypeptidase B2
• NCBI Official Synonym Full Names: carboxypeptidase B2 (plasma)
• NCBI Official Symbol: Cpb2
• NCBI Official Synonym Symbols: CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• NCBI Protein Information: carboxypeptidase B2; carboxypeptidase R; carboxypeptidase U; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
• UniProt Protein Name: Carboxypeptidase B2
• UniProt Gene Name: Cpb2
• UniProt Synonym Gene Names: Tafi; CPR; CPU; TAFI
• UniProt Entry Name: CBPB2_MOUSE

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.20; Secreted, signal peptide; Protease; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; zinc ion binding; metallopeptidase activity; hydrolase activity; carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity

Biological Process: fibrinolysis; negative regulation of fibrinolysis; hemostasis; response to heat; blood coagulation; proteolysis