SDS-Page
Transferrin Receptor (TFR) Recombinant Protein | TFR recombinant protein
Recombinant Transferrin Receptor (TFR)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Transferrin Receptor (TFR); Recombinant Transferrin Receptor (TFR); TFR recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- TKGDTL NLRKIDNLD ALLAAAAEVA GQAALRLTHD HELFLDIGRY SEELLAYQEE FLPYIKEVRE LGLTLDWLFF ARGDFQRAVT ALRRDIANSD GENRVIRRAL NDRMMKVEYD FLSPYLSPKD VPFRHIFFGK GPHTLRSLVE HLQLLKTNRS SVDLNLLREQ LALATWTIKG RPMPWEVISG K
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- TKGDTL NLRKIDNLD ALLAAAAEVA GQAALRLTHD HELFLDIGRY SEELLAYQEE FLPYIKEVRE LGLTLDWLFF ARGDFQRAVT ALRRDIANSD GENRVIRRAL NDRMMKVEYD FLSPYLSPKD VPFRHIFFGK GPHTLRSLVE HLQLLKTNRS SVDLNLLREQ LALATWTIKG RPMPWEVISG K
Sequence Length
2500
Applicable Applications for TFR recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Chicken (Gallus)
Expression System
Prokaryotic expression
Residues
Thr585~Lys771 (Accession # NM_205256.1) with two N-terminal Tags, His-tag and S-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27.2kDa
NCBI Official Full Name
Gallus gallus transferrin receptor (p90, CD71) (TFRC), mRNA
NCBI Official Synonym Full Names
transferrin receptor (p90, CD71)
NCBI Official Symbol
TFRC
NCBI Protein Information
transferrin receptor protein 1; TR; tfR; tfR1; trfr
UniProt Protein Name
Transferrin receptor protein 1
UniProt Gene Name
TFRC
UniProt Synonym Gene Names
TR; TfR; TfR1; Trfr
UniProt Entry Name
TFR1_CHICK
Uniprot Description
Function: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system
By similarity.
Subunit structure: Homodimer; disulfide-linked. Binds one transferrin molecule per subunit
By similarity.
Subcellular location: Membrane; Single-pass type II membrane protein
By similarity.
Post-translational modification: N- and O-glycosylated, phosphorylated and palmitoylated
By similarity.
Sequence similarities: Belongs to the peptidase M28 family. M28B subfamily.Contains 1 PA (protease associated) domain.
Sequence caution: The sequence CAA39035.1 differs from that shown. Reason: Frameshift at position 760. The correct sequence is shown in fig.1 of Ref.1.
Research Articles on TFR
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Product Notes
The TFR tfrc (Catalog #AAA2010017) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Transferrin Receptor (TFR) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the TFR tfrc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- TKGDTL NLRKIDNLD ALLAAAAEVA GQAALRLTHD HELFLDIGRY SEELLAYQEE FLPYIKEVRE LGLTLDWLFF ARGDFQRAVT ALRRDIANSD GENRVIRRAL NDRMMKVEYD FLSPYLSPKD VPFRHIFFGK GPHTLRSLVE HLQLLKTNRS SVDLNLLREQ LALATWTIKG RPMPWEVISG K. It is sometimes possible for the material contained within the vial of "Transferrin Receptor (TFR), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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