Suppressor Of Variegation 4-20 Homolog 2 (SUV420H2) Recombinant Protein | SUV420H2 recombinant protein

Recombinant Suppressor Of Variegation 4-20 Homolog 2 (SUV420H2)

Cat. Num. AAA2009739

• Gene Names: SUV420H2; KMT5C
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Suppressor Of Variegation 4-20 Homolog 2 (SUV420H2); Recombinant Suppressor Of Variegation 4-20 Homolog 2 (SUV420H2); SUV420H2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal His-tag, its sequence is listed below.; MGHHHHHHSGSEF-GPDRVTARE LCENDDLATS LVLDPYLGFR THKMNVSPVP PLRRQQHLRS ALETFLRQRD LEAAYRALTL GGWTARYFQS RGPRQEAALK THVYRYLRAF LPESGFTILP CTRYSMETNG AKIVSTRAWK KNEKLELLVG CIAELREADE GLLRAGENDF SIMYSTRKRS AQLWLGPAAF INHDCKPNCK FVPADGNAAC VKVLRDIEPG DEVTCFYGEG FFGEKNEHCE CHTCERKGEG AFRTRPREPA LPPRPLDKYQ LRETKRRLQQ GLDSGSRQG
• Sequence Length: 462

• Applicable Applications for SUV420H2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly2~Gly279 (Accession # Q86Y97) with a N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31543169
• NCBI GeneID: 84787
• NCBI Accession #: NP_116090.2
• NCBI GenBank Nucleotide #: NM_032701.3
• UniProt Accession #: Q86Y97; Q8WZ10; Q9BRZ6
• Molecular Weight: 33.3kDa
• NCBI Official Full Name: histone-lysine N-methyltransferase SUV420H2
• NCBI Official Synonym Full Names: suppressor of variegation 4-20 homolog 2 (Drosophila)
• NCBI Official Symbol: SUV420H2
• NCBI Official Synonym Symbols: KMT5C
• NCBI Protein Information: histone-lysine N-methyltransferase SUV420H2; suv4-20h2; su(var)4-20 homolog 2; lysine N-methyltransferase 5C
• UniProt Protein Name: Histone-lysine N-methyltransferase SUV420H2
• UniProt Gene Name: SUV420H2
• UniProt Synonym Gene Names: KMT5C; Su(var)4-20 homolog 2; Suv4-20h2
• UniProt Entry Name: SV422_HUMAN

NCBI Description

SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).[supplied by OMIM, Dec 2009]

Uniprot Description

SUV420H2: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: nucleoplasm; centric heterochromatin; nuclear heterochromatin; condensed nuclear chromosome, pericentric region

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H4-K20 specific)

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent