Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) Recombinant Protein | NME1 recombinant protein

Recombinant Non Metastatic Cells 1, Protein NM23A Expressed In (NME1)

Cat. Num. AAA2009667

• Gene Names: Nme1; NM23A; NDPK-A; NM23-M1; AL024257
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Non Metastatic Cells 1; Protein NM23A Expressed In (NME1); Recombinant Non Metastatic Cells 1; NME1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF LQASEDLLKE HYTDLKDRPF FTGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFQPEELV EYKSCAQ
• Sequence Length: 152

• Applicable Applications for NME1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg6~Gln147 (Accession # P15532) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 37700232
• NCBI GeneID: 18102
• NCBI Accession #: NP_032730.1
• NCBI GenBank Nucleotide #: NM_008704.2
• UniProt Accession #: P15532
• Molecular Weight: 19.8kDa
• NCBI Official Full Name: nucleoside diphosphate kinase A
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: Nme1
• NCBI Official Synonym Symbols: NM23A; NDPK-A; NM23-M1; AL024257
• NCBI Protein Information: nucleoside diphosphate kinase A; NDK A; NDP kinase A; nucleotide diphosphate kinase; nucleoside-diphosphate kinase 1; metastasis inhibition factor NM23; expressed in non-metastatic cells 1 protein; tumor metastatic process-associated protein; expressed in non-metastatic cells 1, protein; non-metastatic cells 1, protein (NM23A) expressed in
• UniProt Protein Name: Nucleoside diphosphate kinase A
• Protein Family: Nucleoside diphosphate kinase
• UniProt Gene Name: Nme1
• UniProt Synonym Gene Names: Nm23; NDK A
• UniProt Entry Name: NDKA_MOUSE

Uniprot Description

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.

Protein type: EC 2.7.4.6; Kinase, other; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Protein kinase, histidine; Nucleotide Metabolism - purine; Tumor suppressor; Other group; NDK family

Cellular Component: centrosome; membrane; mitochondrion; perinuclear region of cytoplasm; cytoplasm; intermediate filament; nucleus; cytosol; myelin sheath

Molecular Function: identical protein binding; gamma-tubulin binding; GTP binding; metal ion binding; nucleoside diphosphate kinase activity; intermediate filament binding; magnesium ion binding; nucleotide binding; protein kinase binding; transferase activity; enzyme binding; deoxyribonuclease activity; kinase activity; single-stranded DNA binding; ribosomal small subunit binding; ATP binding

Biological Process: GTP biosynthetic process; lactation; nervous system development; UTP biosynthetic process; negative regulation of myeloid leukocyte differentiation; nucleoside diphosphate phosphorylation; endocytosis; DNA catabolic process; regulation of apoptosis; CTP biosynthetic process; mammary gland development; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; nucleotide metabolic process; cell differentiation; phosphorylation; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; positive regulation of DNA binding