Advanced Glycosylation End Product Specific Receptor (AGER) Recombinant Protein | AGER recombinant protein

Recombinant Advanced Glycosylation End Product Specific Receptor (AGER)

Cat. Num. AAA2009621

• Gene Names: Ager; RAGE
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Advanced Glycosylation End Product Specific Receptor (AGER); Recombinant Advanced Glycosylation End Product Specific Receptor (AGER); AGER recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-W VLVLALWGAV AGGQNITARI GEPLVLSCKG APKKPPQQLE WKLNTGRTEA WKVLSPQGGP WDSVAQILPN GSLLLPATGI VDEGTFRCRA TNRRGKEVKS NYRVRVYQIP GKPEIVDPAS ELTASVPNKV GTCVSEGSYP AGTLSWHLDG KLLIPDGKET LVKEETRRHP ETGLFTLR
• Sequence Length: 403

• Applicable Applications for AGER recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Trp10~Arg188 (Accession # Q62151) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 2497318
• NCBI GeneID: 11596
• UniProt Accession #: Q62151
• Molecular Weight: 23.3kDa
• NCBI Official Full Name: Advanced glycosylation end product-specific receptor
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: Ager
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor; advanced glycation end-products receptor; receptor for advanced glycosylation end products; receptor for advanced glycation end-products variant 20
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: Ager
• UniProt Synonym Gene Names: Rage
• UniProt Entry Name: RAGE_MOUSE

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Membrane protein, integral; Receptor, misc.

Cellular Component: extracellular space; cell surface; cell soma; membrane; axon; cytoplasm; basal plasma membrane; plasma membrane; integral to membrane; cytosol

Molecular Function: identical protein binding; protein binding; advanced glycation end-product receptor activity

Biological Process: positive regulation of JNK activity; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of smooth muscle cell proliferation; negative regulation of cell adhesion; JAK-STAT cascade; positive regulation of smooth muscle cell migration; induction of positive chemotaxis; activation of NF-kappaB transcription factor; calcium ion homeostasis; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; regulation of DNA binding; positive regulation of neuron apoptosis; negative regulation of endothelial cell proliferation; regulation of inflammatory response; response to hypoxia; positive regulation of protein amino acid phosphorylation; inflammatory response; positive regulation of phagocytosis, engulfment; positive regulation of autophagy; neurite development; positive regulation of cell migration; positive regulation of inflammatory response