Interferon Regulatory Factor 1 (IRF1) Recombinant Protein | IRF1 recombinant protein

Recombinant Interferon Regulatory Factor 1 (IRF1)

Cat. Num. AAA2009539

• Gene Names: IRF1; MAR; IRF-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interferon Regulatory Factor 1 (IRF1); Recombinant Interferon Regulatory Factor 1 (IRF1); IRF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN KGSSAVRVYR MLPPLTKNQR KERKSKSSRD AKSKAKRKSC GDSSPDTFSD GLSSSTLPDD HSSYTVPGYM QDLEVEQALT PALSPCAVSS TLPDWHIPVE VVPDSTSDLY NFQVSPMPST SEATTDEDEE GKLPEDIMKL LEQSEWQPTN VDGKGYLLNE PGVQPTSVYG DFSCKE
• Sequence Length: 325

• Applicable Applications for IRF1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Trp11~Glu276 (Accession # P10914) with N-terminal His-Tag
• Subcellular Location: Nucleus. Cytoplasm
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 4504721
• NCBI GeneID: 3659
• NCBI Accession #: NP_002189.1
• NCBI GenBank Nucleotide #: NM_002198.2
• UniProt Accession #: P10914; Q96GG7
• Molecular Weight: 31.5kDa
• NCBI Official Full Name: interferon regulatory factor 1
• NCBI Official Synonym Full Names: interferon regulatory factor 1
• NCBI Official Symbol: IRF1
• NCBI Official Synonym Symbols: MAR; IRF-1
• NCBI Protein Information: interferon regulatory factor 1; interferon regulatory factor 1 isoform d78
• UniProt Protein Name: Interferon regulatory factor 1
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF1
• UniProt Synonym Gene Names: IRF-1
• UniProt Entry Name: IRF1_HUMAN

NCBI Description

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF1: a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon- stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity include: genes involved in anti-viral response, such as IFN-alpha/beta, DDX58, TRAIL, OAS1/2, PIAS1, PKR and RSAD2; antibacterial response, such as iNOS; anti- proliferative response, such as p53, LOX and CDKN1A; apoptosis, such as PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, COX-2 and CYBB; DNA damage responses and DNA repair, such as POLQ; MHC class I expression, such as TAP1, PSMB9, PSME1, PSME2 and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53. Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells. Defects in IRF1 are a cause of gastric adenocarcinoma (GASC), accounting for most of all gastric malignant tumors. Deletions or rearrangements of IRF1 can occur in preleukemic myelodysplastic syndrome (MDS) and acute myelogenous leukemia (AML).

Protein type: Tumor suppressor; Transcription factor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; nucleus; cytosol

Molecular Function: protein binding; DNA binding

Biological Process: transcription from RNA polymerase II promoter; CD8-positive, alpha-beta T cell differentiation; regulation of cell cycle; apoptosis; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; regulation of MyD88-dependent toll-like receptor signaling pathway; regulation of innate immune response; negative regulation of cell proliferation; regulation of adaptive immune response; positive regulation of interleukin-12 biosynthetic process; negative regulation of regulatory T cell differentiation; positive regulation of interferon-beta production; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; cell cycle arrest; negative regulation of transcription, DNA-dependent; defense response to virus

Disease: Gastric Cancer; Lung Cancer