Lens Epithelium Derived Growth Factor (LEDGF) Recombinant Protein | LEDGF recombinant protein

Recombinant Lens Epithelium Derived Growth Factor (LEDGF)

Cat. Num. AAA2009129

• Gene Names: Psip1; Ledgf; Psip2; SBP75
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lens Epithelium Derived Growth Factor (LEDGF); Recombinant Lens Epithelium Derived Growth Factor (LEDGF); LEDGF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DRKRKQEEQM ETEQQTKDEG KKPEVKKVEK KRETSMDSRL QRIHAEIKNS LKIDNLDVNR CIEALDELAS LQVTMQQAQK HTEMITTLKK IRRFKVSQVI MEKSTMLYNK FKNMFLVGEG DSVITQVLNK SLAEQRQHEE ANKTKDQGKK GPNKKLEKE
• Sequence Length: 528

• Applicable Applications for LEDGF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asp311~Glu469 (Accession # Q812D1) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 28461167
• NCBI GeneID: 313323
• NCBI Accession #: NP_786941.1
• NCBI GenBank Nucleotide #: NM_175765.2
• UniProt Accession #: Q812D1; Q7TNY0
• Molecular Weight: 22.5kDa
• NCBI Official Full Name: PC4 and SFRS1-interacting protein
• NCBI Official Synonym Full Names: PC4 and SFRS1 interacting protein 1
• NCBI Official Symbol: Psip1
• NCBI Official Synonym Symbols: Ledgf; Psip2; SBP75
• NCBI Protein Information: PC4 and SFRS1-interacting protein; supercoiled DNA binding protein 75; PC4 and SFRS1 interacting protein 2; lens epithelium-derived growth factor
• UniProt Protein Name: PC4 and SFRS1-interacting protein
• UniProt Gene Name: Psip1
• UniProt Synonym Gene Names: Ledgf
• UniProt Entry Name: PSIP1_RAT

NCBI Description

transcription factor; may mediate survival, adhesiveness, and growth of central lens epithelial cells (LECs) [RGD, Feb 2006]

Uniprot Description

LEDGF: Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN. Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. Belongs to the HDGF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; nucleolus; nuclear heterochromatin; nucleus

Molecular Function: transcription activator binding; chromatin binding

Biological Process: nuclear mRNA 5'-splice site recognition; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to heat; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress