Hypoxia Inducible Factor 2 Alpha (HIF2a) Recombinant Protein | HIF2a recombinant protein

Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a)

Cat. Num. AAA2009050

• Gene Names: EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Hypoxia Inducible Factor 2 Alpha (HIF2a); Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a); HIF2a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-CV NYVLSEIEKN DVVFSMDQTE SLFKPHLMAM NSIFDSSGKG AVSEKSNFLF TKLKEEPEEL AQLAPTPGDA IISLDFGNQN FEESSAYGKA ILPPSQPWAT ELRSHSTQSE AGSLPAFTVP QAAAPGSTTP SATSSSSSCS TPNSPEDYYT SLDNDLKIEV IEKLFAMDTE AKDQCSTQTD FNELDLETLA PYIPMDGEDF Q
• Sequence Length: 870

• Applicable Applications for HIF2a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Cys339~Gln541 (Accession # Q99814) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 40254439
• NCBI GeneID: 2034
• NCBI Accession #: NP_001421.2
• NCBI GenBank Nucleotide #: NM_001430.4
• UniProt Accession #: Q99814; Q86VA2; Q99630
• Molecular Weight: 23.6kDa
• NCBI Official Full Name: endothelial PAS domain-containing protein 1
• NCBI Official Synonym Full Names: endothelial PAS domain protein 1
• NCBI Official Symbol: EPAS1
• NCBI Official Synonym Symbols: HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
• NCBI Protein Information: endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73
• UniProt Protein Name: Endothelial PAS domain-containing protein 1
• UniProt Gene Name: EPAS1
• UniProt Synonym Gene Names: BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha
• UniProt Entry Name: EPAS1_HUMAN

NCBI Description

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

Uniprot Description

HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2p21-p16

Cellular Component: nucleoplasm; transcription factor complex; cytosol

Molecular Function: histone acetyltransferase binding; protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; myoblast cell fate commitment; embryonic placenta development; regulation of heart rate; somatic stem cell maintenance; cell maturation; norepinephrine metabolic process; signal transduction; iron ion homeostasis; visual perception; response to hypoxia; erythrocyte differentiation; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; surfactant homeostasis; lung development

Disease: Erythrocytosis, Familial, 4