TYW5 cdna clone
TYW5
Gene Names
TYW5; hTYW5; C2orf60
Synonyms
TYW5; C2orf60; hTYW5; TYW5 cdna clone
Form/Format
Lyophilized
Sequence
Nucleotide Sequence: ATGGCCGGGCAGCACCTCCCGGTACCCCGGCTGGAGGGCGTTTCTCGGGAGCAGTTCATGCAGCACCTCTACCCACAGAGAAAACCTCTTGTGTTGGAAGGGATTGATTTGGGGCCATGTACAAGCAAATGGACAGTGGATTACCTAAGCCAAGTTGGAGGGAAGAAAGAAGTAAAGATTCATGTTGCTGCAGTTGCACAGATGGACTTCATTAGTAAGAACTTTGTATATAGAACTTTACCTTTTGACCAGTTGGTCCAGAGGGCAGCTGAAGAGAAACATAAAGAATTCTTTGTTTCAGAGGATGAGAAATACTACTTACGGTCACTTGGAGAAGACCCTAGAAAGGATGTTGCAGATATCAGAAAGCAGTTTCCTTTGTTGAAAGGAGATATTAAGTTTCCAGAATTCTTCAAAGAGGAACAGTTCTTTTCCAGTGTTTTTCGAATTAGTTCACCAGGATTACAACTATGGACTCATTATGATGTAATGGATAATTTGTTAATACAAGTGACAGGAAAAAAGCGTGTTGTACTCTTCAGTCCTCGAGATGCCCAGTATTTATATTTAAAAGGTACTAAATCAGAAGTACTGAATATAGATAACCCAGACTTGGCTAAATATCCACTTTTTTCCAAGGCTAGAAGATATGAATGTTCCCTTGAAGCTGGTGATGTATTATTCATTCCTGCTTTATGGTTCCATAATGTAATTTCTGAAGAGTTTGGAGTGGGAGTGAATATCTTTTGGAAGCACCTTCCATCTGAATGCTATGATAAAACAGATACCTATGGAAACAAAGATCCTACAGCAGCATCAAGAGCTGCACAAATTCTGGACAGAGCCTTGAAAACACTGGCCGAGTTACCAGAGGAATATAGGGACTTCTATGCACGACGAATGGTCCTACACATTCAAGACAAAGCCTACAGCAAGAACTCTGAGTAA
Translation Sequence: MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR MVLHIQDKAY SKNSE
Translation Sequence: MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR MVLHIQDKAY SKNSE
Sequence Length
315
Species
Human
Chromosome Location
2q33.1
cDNA Size
939bp
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
Vector
(puc19-derived cloning vector)
Preparation Before Usage
1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
Preparation and Storage
Store the plasmid at -20 degree C.
Related Product Information for TYW5 cdna clone
TYW5 (TRNA-YW Synthesizing Protein 5) acts as a component of the wybutosine biosynthesis pathway. It catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW).
Product Categories/Family for TYW5 cdna clone
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tRNA wybutosine-synthesizing protein 5
NCBI Official Synonym Full Names
tRNA-yW synthesizing protein 5
NCBI Official Symbol
TYW5
NCBI Official Synonym Symbols
hTYW5; C2orf60
NCBI Protein Information
tRNA wybutosine-synthesizing protein 5
UniProt Protein Name
tRNA wybutosine-synthesizing protein 5
Protein Family
UniProt Gene Name
TYW5
UniProt Synonym Gene Names
C2orf60; hTYW5
UniProt Entry Name
TYW5_HUMAN
Uniprot Description
TYW5: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes. Belongs to the TYW5 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.42
Chromosomal Location of Human Ortholog: 2q33.1
Molecular Function: protein homodimerization activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA binding
Biological Process: wybutosine biosynthetic process
Similar Products
Product Notes
The TYW5 tyw5 (Catalog #AAA200871) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Nucleotide Sequence: ATGGCCGGGC AGCACCTCCC GGTACCCCGG CTGGAGGGCG TTTCTCGGGA GCAGTTCATG CAGCACCTCT ACCCACAGAG AAAACCTCTT GTGTTGGAAG GGATTGATTT GGGGCCATGT ACAAGCAAAT GGACAGTGGA TTACCTAAGC CAAGTTGGAG GGAAGAAAGA AGTAAAGATT CATGTTGCTG CAGTTGCACA GATGGACTTC ATTAGTAAGA ACTTTGTATA TAGAACTTTA CCTTTTGACC AGTTGGTCCA GAGGGCAGCT GAAGAGAAAC ATAAAGAATT CTTTGTTTCA GAGGATGAGA AATACTACTT ACGGTCACTT GGAGAAGACC CTAGAAAGGA TGTTGCAGAT ATCAGAAAGC AGTTTCCTTT GTTGAAAGGA GATATTAAGT TTCCAGAATT CTTCAAAGAG GAACAGTTCT TTTCCAGTGT TTTTCGAATT AGTTCACCAG GATTACAACT ATGGACTCAT TATGATGTAA TGGATAATTT GTTAATACAA GTGACAGGAA AAAAGCGTGT TGTACTCTTC AGTCCTCGAG ATGCCCAGTA TTTATATTTA AAAGGTACTA AATCAGAAGT ACTGAATATA GATAACCCAG ACTTGGCTAA ATATCCACTT TTTTCCAAGG CTAGAAGATA TGAATGTTCC CTTGAAGCTG GTGATGTATT ATTCATTCCT GCTTTATGGT TCCATAATGT AATTTCTGAA GAGTTTGGAG TGGGAGTGAA TATCTTTTGG AAGCACCTTC CATCTGAATG CTATGATAAA ACAGATACCT ATGGAAACAA AGATCCTACA GCAGCATCAA GAGCTGCACA AATTCTGGAC AGAGCCTTGA AAACACTGGC CGAGTTACCA GAGGAATATA GGGACTTCTA TGCACGACGA ATGGTCCTAC ACATTCAAGA CAAAGCCTAC AGCAAGAACT CTGAGTAA Tran slation Sequence: MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR MVLHIQDKAY SKNSE. It is sometimes possible for the material contained within the vial of "TYW5, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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