Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat GFAP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Glial Fibrillary Acidic Protein (GFAP) Polyclonal Antibody | anti-GFAP antibody
Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- VYQAELRE LRLRLDQLTT NSARLEVERD NLTQDLGTLR QKLQDETNLR LEAENNLAVY RQEADEATLA RVDLERKVES LEEEIQFLRK IHEEEVRELQ E
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat GFAP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat GFAP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Cerebrum Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Cerebrum Tissue))
NCBI and Uniprot Product Information
NCBI Description
intermediate filament protein specific for astrocytes [RGD, Feb 2006]
Uniprot Description
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 10q32.1
Cellular Component: cell projection; cytoplasm; intermediate filament; intermediate filament cytoskeleton; intracellular; membrane; myelin sheath
Molecular Function: identical protein binding; integrin binding; kinase binding; protein binding; structural constituent of cytoskeleton; structural molecule activity
Biological Process: astrocyte development; Bergmann glial cell differentiation; extracellular matrix organization; intermediate filament organization; intermediate filament-based process; neuron projection regeneration; regulation of neurotransmitter uptake; response to wounding
Research Articles on GFAP
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Product Notes
The GFAP gfap (Catalog #AAA2006798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glial Fibrillary Acidic Protein (GFAP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- VYQAELRE LRLRLDQLTT NSARLEVERD NLTQDLGTLR QKLQDETNLR LEAENNLAVY RQEADEATLA RVDLERKVES LEEEIQFLRK IHEEEVRELQ E. It is sometimes possible for the material contained within the vial of "Glial Fibrillary Acidic Protein (GFAP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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