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Western Blot (WB)
(Western Blot: Sample: Recombinant APOH, Mouse.)
Rabbit anti-Mouse Apolipoprotein H (APOH) Polyclonal Antibody | anti-APOH antibody
Polyclonal Antibody to Apolipoprotein H (APOH)
Gene Names
Apoh; B2GPI; beta2-GPI; beta-2-GPI
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Apolipoprotein H (APOH); Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein H (APOH); anti-APOH antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against APOH. It has been selected for its ability to recognize APOH in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-RICPKPDDLP FATVVPLKTS YDPGEQIVYS CKPGYVSRGG MRRFTCPLTG MWPINTLRCV PRVCPFAGIL ENGIVRYTSF EYPKNISFAC NPGFFLNGTS SSKCTEEGKW SPDIPACARI TCPPPPVPKF ALLKDYRPSA GNNSLYQDTV VFKCLPHFAM IGNDTVMCTE QGNWTRLPEC LEVKCPFPPR PENGYVNYPA KPVLLYKDKA TFGCHETYKL DGPEEAECTK TGTWSFLPTC RESCKLPVKK ATVLYQGMRV KIQEQFKNGM MHGDKIHFYC KNKEKKCSYT VEAHCRDGTI EIPSCFKEHS SLAFWKTDAS ELTPC
MGHHHHHHSGSEF-RICPKPDDLP FATVVPLKTS YDPGEQIVYS CKPGYVSRGG MRRFTCPLTG MWPINTLRCV PRVCPFAGIL ENGIVRYTSF EYPKNISFAC NPGFFLNGTS SSKCTEEGKW SPDIPACARI TCPPPPVPKF ALLKDYRPSA GNNSLYQDTV VFKCLPHFAM IGNDTVMCTE QGNWTRLPEC LEVKCPFPPR PENGYVNYPA KPVLLYKDKA TFGCHETYKL DGPEEAECTK TGTWSFLPTC RESCKLPVKK ATVLYQGMRV KIQEQFKNGM MHGDKIHFYC KNKEKKCSYT VEAHCRDGTI EIPSCFKEHS SLAFWKTDAS ELTPC
Sequence Length
345
Applicable Applications for anti-APOH antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant APOH (Arg21~Cys345) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040961
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant APOH, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant APOH, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,619 Da
NCBI Official Full Name
beta-2-glycoprotein 1
NCBI Official Synonym Full Names
apolipoprotein H
NCBI Official Symbol
Apoh
NCBI Official Synonym Symbols
B2GPI; beta2-GPI; beta-2-GPI
NCBI Protein Information
beta-2-glycoprotein 1
UniProt Protein Name
Beta-2-glycoprotein 1
Protein Family
UniProt Gene Name
Apoh
UniProt Synonym Gene Names
B2gp1; Apo-H; B2GPI; Beta(2)GPI
Uniprot Description
APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Protein type: Lipid-binding; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11 E1|11 71.8 cM
Cellular Component: cell surface; chylomicron; cytoplasm; extracellular matrix; extracellular region; extracellular space; plasma membrane
Molecular Function: heparin binding; identical protein binding; lipid binding; phospholipid binding
Biological Process: blood coagulation; blood coagulation, intrinsic pathway; negative regulation of angiogenesis; negative regulation of blood coagulation; negative regulation of endothelial cell proliferation; negative regulation of fibrinolysis; negative regulation of myeloid cell apoptosis; organ regeneration; plasminogen activation; positive regulation of lipoprotein lipase activity; regulation of blood coagulation; regulation of fibrinolysis; triacylglycerol metabolic process; triglyceride transport
Research Articles on APOH
Similar Products
Product Notes
The APOH apoh (Catalog #AAA2006705) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apolipoprotein H (APOH) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein H (APOH) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the APOH apoh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-RICPKP DDLP FATVVPLKTS YDPGEQIVYS CKPGYVSRGG MRRFTCPLTG MWPINTLRCV PRVCPFAGIL ENGIVRYTSF EYPKNISFAC NPGFFLNGTS SSKCTEEGKW SPDIPACARI TCPPPPVPKF ALLKDYRPSA GNNSLYQDTV VFKCLPHFAM IGNDTVMCTE QGNWTRLPEC LEVKCPFPPR PENGYVNYPA KPVLLYKDKA TFGCHETYKL DGPEEAECTK TGTWSFLPTC RESCKLPVKK ATVLYQGMRV KIQEQFKNGM MHGDKIHFYC KNKEKKCSYT VEAHCRDGTI EIPSCFKEHS SLAFWKTDAS ELTPC. It is sometimes possible for the material contained within the vial of "Apolipoprotein H (APOH), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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