Apolipoprotein H (APOH) Recombinant Protein | APOH recombinant protein

Recombinant Apolipoprotein H (APOH)

Cat. Num. AAA2009964

• Gene Names: Apoh; B2GPI; beta2-GPI; beta-2-GPI
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apolipoprotein H (APOH); Recombinant Apolipoprotein H (APOH); APOH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RICPKPDDLP FATVVPLKTS YDPGEQIVYS CKPGYVSRGG MRRFTCPLTG MWPINTLRCV PRVCPFAGIL ENGIVRYTSF EYPKNISFAC NPGFFLNGTS SSKCTEEGKW SPDIPACARI TCPPPPVPKF ALLKDYRPSA GNNSLYQDTV VFKCLPHFAM IGNDTVMCTE QGNWTRLPEC LEVKCPFPPR PENGYVNYPA KPVLLYKDKA TFGCHETYKL DGPEEAECTK TGTWSFLPTC RESCKLPVKK ATVLYQGMRV KIQEQFKNGM MHGDKIHFYC KNKEKKCSYT VEAHCRDGTI EIPSCFKEHS SLAFWKTDAS ELTPC
• Sequence Length: 345

• Applicable Applications for APOH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg21~Cys345 (Accession # Q01339) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 160358825
• NCBI GeneID: 11818
• NCBI Accession #: NP_038503.4
• NCBI GenBank Nucleotide #: NM_013475.4
• UniProt Accession #: Q01339
• Molecular Weight: 38.1kDa
• NCBI Official Full Name: beta-2-glycoprotein 1
• NCBI Official Synonym Full Names: apolipoprotein H
• NCBI Official Symbol: Apoh
• NCBI Official Synonym Symbols: B2GPI; beta2-GPI; beta-2-GPI
• NCBI Protein Information: beta-2-glycoprotein 1; apo-H; beta(2)GPI; APC inhibitor; beta-2-glycoprotein I; activated protein C-binding protein
• UniProt Protein Name: Beta-2-glycoprotein 1
• Protein Family: Beta-2-glycoprotein
• UniProt Gene Name: Apoh
• UniProt Synonym Gene Names: B2gp1; Apo-H; B2GPI; Beta(2)GPI
• UniProt Entry Name: APOH_MOUSE

Uniprot Description

APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Protein type: Lipid-binding; Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; extracellular region

Molecular Function: heparin binding; identical protein binding; phospholipid binding; glycoprotein binding; lipid binding

Biological Process: organ regeneration; negative regulation of blood coagulation; negative regulation of angiogenesis; negative regulation of myeloid cell apoptosis; negative regulation of fibrinolysis; triacylglycerol metabolic process; negative regulation of endothelial cell proliferation; regulation of blood coagulation; positive regulation of lipoprotein lipase activity; triacylglycerol transport; blood coagulation; blood coagulation, intrinsic pathway; plasminogen activation; regulation of fibrinolysis