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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Colony Stimulating Factor Receptor, Granulocyte (GCSFR) Polyclonal Antibody | anti-GCSFR antibody

Polyclonal Antibody to Colony Stimulating Factor Receptor, Granulocyte (GCSFR)

Gene Names
Csf3r; Cd114; Csfgr; G-CSFR; G-CSF-R
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Colony Stimulating Factor Receptor; Granulocyte (GCSFR); Polyclonal Antibody; Polyclonal Antibody to Colony Stimulating Factor Receptor; anti-GCSFR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GCSFR. It has been selected for its ability to recognize GCSFR in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-KTSFWS DVPDPAHSSL SSWLPTIMTE ETFQLPSFWD SSVPSITKIT ELEEDKKPTH WDSESSGNGS LPALVQAYVL QGDPREISNQ SQPPSRTGDQ VLYGQVLESP TSPGVMQYIR SDSTQPLLGG PTPSPKSYEN IWFHSRPQET FVPQPPNQED DCVFGPPFDF PLFQGLQVHG VEEQGGF
Sequence Length
224
Applicable Applications for anti-GCSFR antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GCSFR (Lys655~Phe837) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2055594
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
93,379 Da
NCBI Official Full Name
granulocyte colony-stimulating factor receptor isoform 1
NCBI Official Synonym Full Names
colony stimulating factor 3 receptor (granulocyte)
NCBI Official Symbol
Csf3r
NCBI Official Synonym Symbols
Cd114; Csfgr; G-CSFR; G-CSF-R
NCBI Protein Information
granulocyte colony-stimulating factor receptor
UniProt Protein Name
Granulocyte colony-stimulating factor receptor
UniProt Gene Name
Csf3r
UniProt Synonym Gene Names
Csfgr; G-CSF receptor; G-CSF-R

Uniprot Description

GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 4 D2.2|4 59.77 cM

Cellular Component: integral component of membrane; membrane

Molecular Function: granulocyte colony-stimulating factor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding

Biological Process: cell adhesion; neutrophil chemotaxis; regulation of myeloid cell differentiation

Research Articles on GCSFR

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Product Notes

The GCSFR csf3r (Catalog #AAA2005990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Colony Stimulating Factor Receptor, Granulocyte (GCSFR) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Colony Stimulating Factor Receptor, Granulocyte (GCSFR) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GCSFR csf3r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-KTSFWS DVPDPAHSSL SSWLPTIMTE ETFQLPSFWD SSVPSITKIT ELEEDKKPTH WDSESSGNGS LPALVQAYVL QGDPREISNQ SQPPSRTGDQ VLYGQVLESP TSPGVMQYIR SDSTQPLLGG PTPSPKSYEN IWFHSRPQET FVPQPPNQED DCVFGPPFDF PLFQGLQVHG VEEQGGF. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor Receptor, Granulocyte (GCSFR), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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