Western Blot (WB)
(Western Blot: Sample: Recombinant KRT5, Mouse.)
Rabbit anti-Mouse Keratin 5 (KRT5) Polyclonal Antibody | anti-KRT5 antibody
Polyclonal Antibody to Keratin 5 (KRT5)
Gene Names
Krt5; K5; CK5; Tfip8; Krt2-5; AW146334; 3300001P10Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Keratin 5 (KRT5); Polyclonal Antibody; Polyclonal Antibody to Keratin 5 (KRT5); anti-KRT5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT5. It has been selected for its ability to recognize KRT5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.45mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-REQIKTLN NKFASFIDKV RFLEQQNKVL DTKWALLQEQ GTKTIKQNLD PLFEQYINNL RRQLDGVLGE RGRLDSELRN MQDLVEDYKN KYEDEINKRT TAENEFVMLK KDVDAAYMNK VELEARVDAL MDEINFMKMF FDAELSQMQT HVSDTSVVLS MDNNRSLDLD SIIAEVKAQY EDIANRSRTE AESWYQTKYE ELQQTAGRHG DDLRNTKHEI SEMNRMIQRL RSEIDNVKKQ CANLQNAIAE AEQRGELALK DARNKLTELE EALQKAKQDM ARLLREYQEL MNTKLALDVE IATYRKLLEG E
MGHHHHHHSGSEF-REQIKTLN NKFASFIDKV RFLEQQNKVL DTKWALLQEQ GTKTIKQNLD PLFEQYINNL RRQLDGVLGE RGRLDSELRN MQDLVEDYKN KYEDEINKRT TAENEFVMLK KDVDAAYMNK VELEARVDAL MDEINFMKMF FDAELSQMQT HVSDTSVVLS MDNNRSLDLD SIIAEVKAQY EDIANRSRTE AESWYQTKYE ELQQTAGRHG DDLRNTKHEI SEMNRMIQRL RSEIDNVKKQ CANLQNAIAE AEQRGELALK DARNKLTELE EALQKAKQDM ARLLREYQEL MNTKLALDVE IATYRKLLEG E
Sequence Length
590
Applicable Applications for anti-KRT5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant KRT5 (Arg163~Glu471) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043004
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant KRT5, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant KRT5, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Skin Tissue; Lane2: Rat Tongue Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Skin Tissue; Lane2: Rat Tongue Tissue.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,767 Da
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
Krt5
NCBI Official Synonym Symbols
K5; CK5; Tfip8; Krt2-5; AW146334; 3300001P10Rik
NCBI Protein Information
keratin, type II cytoskeletal 5
UniProt Protein Name
Keratin, type II cytoskeletal 5
Protein Family
UniProt Gene Name
Krt5
UniProt Synonym Gene Names
CK-5; K5
Uniprot Description
K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 15 F2|15 56.98 cM
Cellular Component: cytoplasm; extracellular exosome; intermediate filament; keratin filament; membrane; mitochondrion; nucleus; plasma membrane
Molecular Function: protein binding; structural molecule activity
Research Articles on KRT5
Similar Products
Product Notes
The KRT5 krt5 (Catalog #AAA2005414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Keratin 5 (KRT5) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 5 (KRT5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the KRT5 krt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-REQIKT LN NKFASFIDKV RFLEQQNKVL DTKWALLQEQ GTKTIKQNLD PLFEQYINNL RRQLDGVLGE RGRLDSELRN MQDLVEDYKN KYEDEINKRT TAENEFVMLK KDVDAAYMNK VELEARVDAL MDEINFMKMF FDAELSQMQT HVSDTSVVLS MDNNRSLDLD SIIAEVKAQY EDIANRSRTE AESWYQTKYE ELQQTAGRHG DDLRNTKHEI SEMNRMIQRL RSEIDNVKKQ CANLQNAIAE AEQRGELALK DARNKLTELE EALQKAKQDM ARLLREYQEL MNTKLALDVE IATYRKLLEG E. It is sometimes possible for the material contained within the vial of "Keratin 5 (KRT5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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