Rabbit anti-Human Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Polyclonal Antibody | anti-PCK1 antibody

Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)

Cat. Num. AAA2005396

• Gene Names: PCK1; PCKDC; PEPCK1; PEPCKC; PEPCK-C
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Phosphoenolpyruvate Carboxykinase 1; Soluble (PCK1); Polyclonal Antibody; Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1; anti-PCK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against PCK1. It has been selected for its ability to recognize PCK1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-DIAWMKFDAQ GHLRAINPEN GFFGVAPGTS VKTNPNAIKT IQKNTIFTNV AETSDGGVYW EGIDEPLASG VTITSWKNKE WSSEDGEPCA HPNSRFCTPA SQCPIIDAAW ESPEGVPIEG IIFGGRRPAG VPLVYEALSW QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM AQHPAAKLPK IFHVNWFRKD KEGKFLWPGF GENSRVLEWM FNRIDGKAST KLTPIGYI

• Applicable Applications for anti-PCK1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant PCK1 (Asp311~Ile558) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2049672
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 187281517
• NCBI GeneID: 5105
• NCBI Accession #: NP_002582.3
• NCBI GenBank Nucleotide #: NM_002591.3
• UniProt Accession #: P35558; Q8TCA3; Q9UJD2; A8K437; B4DT64
• Molecular Weight: 34,156 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase, cytosolic
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 1
• NCBI Official Symbol: PCK1
• NCBI Official Synonym Symbols: PCKDC; PEPCK1; PEPCKC; PEPCK-C
• NCBI Protein Information: phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Protein Family: Protein kinase
• UniProt Gene Name: PCK1
• UniProt Synonym Gene Names: PEPCK-C

NCBI Description

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 4.1.1.32; Kinase, other; Lyase

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol

Molecular Function: carboxylic acid binding; GDP binding; GTP binding; magnesium ion binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity

Biological Process: aging; cellular response to insulin stimulus; cellular response to potassium ion starvation; gluconeogenesis; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process from pyruvate; internal protein amino acid acetylation; oxaloacetate metabolic process; response to activity; response to insulin stimulus; response to lipopolysaccharide

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic