Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Stem Cell Factor (SCF) Polyclonal Antibody | anti-SCF antibody
Polyclonal Antibody to Stem Cell Factor (SCF)
MGHHHHHHSG S- EICR NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVACMEENAP KNVKESLKKP ETRNFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNE
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue))
NCBI and Uniprot Product Information
NCBI Description
soluble growth factor that activates c-kit tyrosine kinase receptor; important for signaling in spermatogenesis; putative regulator of Leydig cell development [RGD, Feb 2006]
Uniprot Description
SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q21
Cellular Component: cytoplasm; cytoskeleton; extracellular space; filopodium; integral component of membrane; lamellipodium; plasma membrane
Molecular Function: cytokine activity; growth factor activity; stem cell factor receptor binding
Biological Process: cell adhesion; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; negative regulation of apoptosis; negative regulation of mast cell apoptosis; neural crest cell migration; ovarian follicle development; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of leukocyte migration; positive regulation of MAP kinase activity; positive regulation of melanocyte differentiation; positive regulation of myeloid leukocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of Ras protein signal transduction
Research Articles on SCF
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Product Notes
The SCF kitlg (Catalog #AAA2005092) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Stem Cell Factor (SCF) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Stem Cell Factor (SCF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SCF kitlg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S- EICR NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVACMEENAP KNVKESLKKP ETRNFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNE. It is sometimes possible for the material contained within the vial of "Stem Cell Factor (SCF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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