Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) Polyclonal Antibody | anti-ALDH7A1 antibody
Polyclonal Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)
MGHHHHHHSGS- LMS TLLIHHPQYA WLQDLGLRED NEGVFNGSWG GRGEVITTYC PANNEPIARV RQASMKDYEE TIGKAKKAWN IWADIPAPKR GEIVRKIGDA LREKIQLLGR LVSLEMGKIL VEGIGEVQEY VDVCDYAAGL SRMIGGPTLP SERPGHALME QWNPLGLVGI ITAFNFPVAV FGWNNAIALI TGNVCLWKGA PTTSLVSIAV TKIIAKVLED NLLPGAICSL TCGGADMGTA MARDERVNLL SFTGSTQVGK QVALMVQERF GKSLLELGGN NAIIAFEDAD LSLVLPSALF AAVGTAGQRC TTVRRLFLHE SIHDEVVDRL KNAYSQIRVG NPWDPNILYG PLHTKQAVSM FVQAVEEAKK EGGTVVYGGK VMDHPGNYVE PTIVTGLVHD APIVHKETFA PILYVFKFKN EEEVFEWNNE VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRS TCTINYSTAL PLAQGIKFQ
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. It is also involved in lysine catabolism that is known to occur in the mitochondrial matrix. Recent reports show that this protein is found both in the cytosol and the mitochondria, and the two forms likely arise from the use of alternative translation initiation sites. An additional variant encoding a different isoform has also been found for this gene. Mutations in this gene are associated with pyridoxine-dependent epilepsy. Several related pseudogenes have also been identified. [provided by RefSeq, Jan 2011]
Uniprot Description
ALDH7A1: Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism. Defects in ALDH7A1 are the cause of pyridoxine-dependent epilepsy (PDE). PDE is characterized by a combination of various seizure types. It usually occurs in the first hours of life and is unresponsive to standard anticonvulsants, responding only to immediate administration of pyridoxine hydrochloride. Belongs to the aldehyde dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - histidine; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - ascorbate and aldarate; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - butanoate; Mitochondrial; EC 1.2.1.31; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - propanoate; EC 1.2.1.8; Amino Acid Metabolism - arginine and proline; EC 1.2.1.3
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol
Molecular Function: L-aminoadipate-semialdehyde dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; protein binding; betaine-aldehyde dehydrogenase activity
Biological Process: sensory perception of sound; glycine betaine biosynthetic process from choline; lysine catabolic process; aldehyde metabolic process
Disease: Epilepsy, Pyridoxine-dependent
Research Articles on ALDH7A1
Similar Products
Product Notes
The ALDH7A1 aldh7a1 (Catalog #AAA2004430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ALDH7A1 aldh7a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S- LMS TLLIHHPQYA WLQDLGLRED NEGVFNGSWG GRGEVITTYC PANNEPIARV RQASMKDYEE TIGKAKKAWN IWADIPAPKR GEIVRKIGDA LREKIQLLGR LVSLEMGKIL VEGIGEVQEY VDVCDYAAGL SRMIGGPTLP SERPGHALME QWNPLGLVGI ITAFNFPVAV FGWNNAIALI TGNVCLWKGA PTTSLVSIAV TKIIAKVLED NLLPGAICSL TCGGADMGTA MARDERVNLL SFTGSTQVGK QVALMVQERF GKSLLELGGN NAIIAFEDAD LSLVLPSALF AAVGTAGQRC TTVRRLFLHE SIHDEVVDRL KNAYSQIRVG NPWDPNILYG PLHTKQAVSM FVQAVEEAKK EGGTVVYGGK VMDHPGNYVE PTIVTGLVHD APIVHKETFA PILYVFKFKN EEEVFEWNNE VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRS TCTINYSTAL PLAQGIKFQ. It is sometimes possible for the material contained within the vial of "Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
