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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Bone Marrow Stromal Cell Antigen 1 (BST1) Polyclonal Antibody | anti-BST1 antibody

Polyclonal Antibody to Bone Marrow Stromal Cell Antigen 1 (BST1)

Gene Names
Bst1; Bp3; BP-3; Ly65; Bsta1; CD157; 114/A10; A530073F09
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Bone Marrow Stromal Cell Antigen 1 (BST1); Polyclonal Antibody; Polyclonal Antibody to Bone Marrow Stromal Cell Antigen 1 (BST1); anti-BST1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.38mg/ml (varies by lot)
Applicable Applications for anti-BST1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2 ug/mL;1:190-760
Immunohistochemistry: 5-20 ug/mL;1:19-76
Immunocytochemistry: 5-20 ug/mL;1:19-76

Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant BST1 (Arg28~Met282 (Accession #Q64277)) expressed in E.coli
Cross Reactivity
Mouse
Preparation and Storage
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
NCBI Official Synonym Full Names
bone marrow stromal cell antigen 1
NCBI Official Symbol
Bst1
NCBI Official Synonym Symbols
Bp3; BP-3; Ly65; Bsta1; CD157; 114/A10; A530073F09
NCBI Protein Information
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
UniProt Protein Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
Protein Family
UniProt Gene Name
Bst1
UniProt Synonym Gene Names
Bp-3; Bp3; Ly65; BST-1; cADPr hydrolase 2; Ly-65

Uniprot Description

BST1: Synthesizes cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. Belongs to the ADP-ribosyl cyclase family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.2.2.6; Hydrolase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5 B3|5 23.84 cM

Cellular Component: extracellular exosome; extrinsic component of membrane; integrin complex; membrane; plasma membrane; uropod

Molecular Function: ADP-ribosyl cyclase activity; cyclic ADP-ribose hydrolase; hydrolase activity; NAD(P)+ nucleosidase activity; NAD+ nucleosidase activity; NAD+ nucleotidase, cyclic ADP-ribose generating; phosphorus-oxygen lyase activity; transferase activity

Biological Process: positive regulation of B cell proliferation; positive regulation of cell proliferation; regulation of actin cytoskeleton organization; regulation of calcium-mediated signaling; regulation of cell-matrix adhesion; regulation of inflammatory response; regulation of neutrophil chemotaxis; regulation of peptidyl-tyrosine phosphorylation

Research Articles on BST1

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Product Notes

The BST1 bst1 (Catalog #AAA2004396) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Bone Marrow Stromal Cell Antigen 1 (BST1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Bone Marrow Stromal Cell Antigen 1 (BST1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:190-760 Immunohistochemistry: 5-20 ug/mL;1:19-76 Immunocytochemistry: 5-20 ug/mL;1:19-76 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the BST1 bst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bone Marrow Stromal Cell Antigen 1 (BST1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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