Western Blot (WB)
(The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombnant protein (50ng) Lane 2. : HeLa cell lysate Lane 3. : HepG2 cell lysate Lane 4. : 293T cell lysate Lane 5. : Jurkat cell lysate Lane 6. : NIH3T3 cell lysate Lane 7. : A549 cell lysate Lane 8. : MCF7 cell lysate )
Mouse anti-Human PGAM2 Monoclonal Antibody | anti-PGAM2 antibody
PGAM2 antibody
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombnant protein (50ng) Lane 2. : HeLa cell lysate Lane 3. : HepG2 cell lysate Lane 4. : 293T cell lysate Lane 5. : Jurkat cell lysate Lane 6. : NIH3T3 cell lysate Lane 7. : A549 cell lysate Lane 8. : MCF7 cell lysate )
Western Blot (WB)
(The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombnant protein (50ng) Lane 2. : HeLa cell lysate Lane 3. : HepG2 cell lysate Lane 4. : 293T cell lysate Lane 5. : Jurkat cell lysate Lane 6. : NIH3T3 cell lysate Lane 7. : A549 cell lysate Lane 8. : MCF7 cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of PGAM2 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGAM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of PGAM2 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGAM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]
Uniprot Description
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
Research Articles on PGAM2
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Product Notes
The PGAM2 pgam2 (Catalog #AAA200398) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PGAM2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PGAM2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the PGAM2 pgam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGAM2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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