Rabbit anti-Human, Mouse PGAM2 Polyclonal Antibody | anti-PGAM2 antibody

PGAM2, NT (PGAM2, PGAMM, Phosphoglycerate mutase 2, BPG-dependent PGAM 2, Muscle-specific phosphoglycerate mutase, Phosphoglycerate mutase isozyme M) (Azide free) (HRP)

Cat. Num. AAA6332951

• Gene Names: PGAM2; GSD10; PGAMM; PGAM-M
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGAM2; Polyclonal Antibody; NT (PGAM2; PGAMM; Phosphoglycerate mutase 2; BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M) (Azide free) (HRP); Phosphoglycerate mutase isozyme M; anti-PGAM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PGAM2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PGAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human PGAM2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PGAM2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PGAM2 antibody detected the PGAM2 protein (arrow).)

Western Blot (WB)

(PGAM2 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PGAM2 antibody detected the PGAM2 protein (arrow).)

Immunohistochemistry (IHC)

(PGAM2 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PGAM2 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PGAM2 antibody

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). PGAM2 encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X.

Product Categories/Family for anti-PGAM2 antibody

Antibodies; Enzymes

References

Hadjigeorgiou, G.M., et al. Neuromuscul. Disord. 9 (6-7), 399-402 (1999): Tsujino, S., et al. Am. J. Hum. Genet. 52(3):472-477(1993) Castella-Escola, J., et al. Gene 91(2):225-232(1990) Castella-Escola, J., et al. Hum. Genet. 84(2):210-212(1990) Tsujino, S., et al. J. Biol. Chem. 264(26):15334-15337(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 50593010
• NCBI GeneID: 5224
• NCBI Accession #: NP_000281.2
• NCBI GenBank Nucleotide #: NM_000290.3
• UniProt Accession #: P15259
• Molecular Weight: 28,766 Da
• NCBI Official Full Name: phosphoglycerate mutase 2
• NCBI Official Synonym Full Names: phosphoglycerate mutase 2 (muscle)
• NCBI Official Symbol: PGAM2
• NCBI Official Synonym Symbols: GSD10; PGAMM; PGAM-M
• NCBI Protein Information: phosphoglycerate mutase 2; BPG-dependent PGAM 2; muscle-specific phosphoglycerate mutase; phosphoglycerate mutase isozyme M
• UniProt Protein Name: Phosphoglycerate mutase 2
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: PGAM2
• UniProt Synonym Gene Names: PGAMM; PGAM-M
• UniProt Entry Name: PGAM2_HUMAN

NCBI Description

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]

Uniprot Description

PGAM2: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); Isomerase; EC 5.4.2.11; EC 5.4.2.4; EC 3.1.3.13

Chromosomal Location of Human Ortholog: 7p13-p12

Cellular Component: nucleus; cytosol

Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; cofactor binding; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity

Biological Process: response to mercury ion; striated muscle contraction; glycolysis; dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; spermatogenesis; gluconeogenesis

Disease: Glycogen Storage Disease X