Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (The Recombinant human GOT2 (50ng), Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GOT2 Recombinant protein Lane 2.: Ramos cell lysate Lane 3.: 293T cell lysate Lane 4.: Liver tissue lysate The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Balb/3T3 cell lysate The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GOT1 Lane 2.: Recombinant Human GOT2)

Mouse anti-Human GOT2 Monoclonal Antibody | anti-GOT2 antibody

GOT2 antibody

Gene Names
GOT2; KAT4; KATIV; KYAT4; mitAAT
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
GOT2; Monoclonal Antibody; GOT2 antibody; Aspartate aminotransferase 2 mitochondrial; Aspartate aminotransferase 2; mitochondrial; KAT4; KATIV; mitAAT; anti-GOT2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG3, k
Clone Number
AT8B11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
430
Applicable Applications for anti-GOT2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
ICC/IF: 1:100.
Antigen Species
Human
Immunogen
Recombinant human GOT2 (30-430aa) purified from E Coli.
Clone Note
Anti-human GOT2 mAb, clone AT8B11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GOT2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Recombinant human GOT2 (50ng), Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GOT2 Recombinant protein Lane 2.: Ramos cell lysate Lane 3.: 293T cell lysate Lane 4.: Liver tissue lysate The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Balb/3T3 cell lysate The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GOT1 Lane 2.: Recombinant Human GOT2)

Western Blot (WB) (The Recombinant human GOT2 (50ng), Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GOT2 Recombinant protein Lane 2.: Ramos cell lysate Lane 3.: 293T cell lysate Lane 4.: Liver tissue lysate The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Balb/3T3 cell lysate The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GOT1 Lane 2.: Recombinant Human GOT2)

Immunofluorescence (IF)

(ICC/IF analysis of GOT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of GOT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-GOT2 antibody
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. Also, GOT2 is a major participant in the malate-aspartate shuttle, which is a passage from the cytosol to the mitochondria. The two enzymes are homodimeric and show close homology. GOT2 has been seen to have a role in cell proliferation, especially in terms of tumor growth.
Product Categories/Family for anti-GOT2 antibody
References
Yang H., et al. (2015) The EMBO journal. 34 (8): 1110-1125.; Guidetti P., et al. (2007) Journal of neurochemistry. 102 (1): 103-111.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,030 Da
NCBI Official Full Name
aspartate aminotransferase, mitochondrial isoform 1
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 2
NCBI Official Symbol
GOT2
NCBI Official Synonym Symbols
KAT4; KATIV; KYAT4; mitAAT
NCBI Protein Information
aspartate aminotransferase, mitochondrial
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
UniProt Gene Name
GOT2
UniProt Synonym Gene Names
mAspAT; FABP-1; FABPpm

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.

Research Articles on GOT2

Similar Products

Product Notes

The GOT2 got2 (Catalog #AAA200338) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GOT2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GOT2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000 ICC/IF: 1:100. Researchers should empirically determine the suitability of the GOT2 got2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOT2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.