Rabbit anti-Mouse Nitric Oxide Synthase 1, Neuronal (NOS1) Polyclonal Antibody | anti-NOS1 antibody

Polyclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1)

Cat. Num. AAA2003334

• Gene Names: Nos1; NO; NOS; bNOS; nNOS; N-NOS; NOS-I; Nos-1; NC-NOS; 2310005C01Rik
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Nitric Oxide Synthase 1; Neuronal (NOS1); Polyclonal Antibody; Polyclonal Antibody to Nitric Oxide Synthase 1; anti-NOS1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against NOS1. It has been selected for its ability to recognize NOS1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-FHQEMLNYRL TPSFEYQPDP WNTHVWKGTN GTPTKRRAIG FKKLAEAVKF SAKLMGQAMA KRVKATILYA TETGKSQAYA KTLCEIFKHA FDAKAMSMEE YDIVHLEHEA LVLVVTSTFG NGDPPENGEK FGCALM
• Sequence Length: 1434

• Applicable Applications for anti-NOS1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant NOS1 (Phe691~Met826) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2047840
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 6724321
• NCBI GeneID: 18125
• NCBI Accession #: NP_032738.1
• NCBI GenBank Nucleotide #: NM_008712.3
• UniProt Accession #: Q9Z0J4; Q3UR10; Q64208
• Molecular Weight: 164,343 Da
• NCBI Official Full Name: nitric oxide synthase, brain
• NCBI Official Synonym Full Names: nitric oxide synthase 1, neuronal
• NCBI Official Symbol: Nos1
• NCBI Official Synonym Symbols: NO; NOS; bNOS; nNOS; N-NOS; NOS-I; Nos-1; NC-NOS; 2310005C01Rik
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos1
• UniProt Synonym Gene Names: N-NOS; nNOS

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 5 F|5 57.29 cM

Cellular Component: azurophil granule; caveola; cell projection; cytoplasm; cytoskeleton; cytosol; dendrite; membrane; membrane raft; mitochondrial outer membrane; mitochondrion; nuclear membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; photoreceptor inner segment; plasma membrane; postsynaptic density; protein complex; sarcolemma; sarcoplasmic reticulum; sarcoplasmic reticulum membrane; synapse; T-tubule; vesicle membrane; Z disc

Molecular Function: ATPase binding; cadmium ion binding; calmodulin binding; enzyme binding; FAD binding; FMN binding; heme binding; ion channel binding; iron ion binding; metal ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; oxidoreductase activity; phosphoprotein binding; protein binding; protein homodimerization activity; sodium channel regulator activity

Biological Process: arginine catabolic process; behavioral response to cocaine; cellular response to epinephrine stimulus; cellular response to growth factor stimulus; establishment of protein localization; exogenous drug catabolic process; multicellular organismal response to stress; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of heart contraction; negative regulation of hydrolase activity; negative regulation of insulin secretion; negative regulation of peptidyl-serine phosphorylation; negative regulation of potassium ion transport; negative regulation of serotonin uptake; negative regulation of vasoconstriction; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of adrenergic receptor signaling pathway involved in heart process; positive regulation of blood vessel diameter; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of long-term synaptic potentiation; positive regulation of neuron death; positive regulation of sodium ion transmembrane transport; positive regulation of the force of heart contraction; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; reduction of cytosolic calcium ion concentration; regulation of heart contraction; regulation of neurogenesis; regulation of sensory perception of pain; regulation of sodium ion transport; response to estrogen; response to heat; response to peptide hormone; retrograde trans-synaptic signaling by nitric oxide; striated muscle contraction; vasodilation