Western Blot (WB)
(Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Human Smad3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Mothers Against Decapentaplegic Homolog 3 (Smad3) Polyclonal Antibody | anti-Smad3 antibody
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)
MGHHHHHHSG SEF- FWCSISYYEL NQRVGETFHA SQPSMTVDGF TDPSNSERFC LGLLSNVNRN AAVELTRRHI GRGVRLYYIG GEVFAECLSD SAIFVQSPNC NQRYGWHPAT VCKIPPGCNL KIFNNQEFAA LLAQSVNQGF EAVYQLTRMC TIRMSFVKGW GAEYRRQTVT
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Human Smad3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Human Smad3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: Smad3 knockout PC3 cell lysate; Predicted MW: 26,36,43,48kd Observed MW: 43kd Primary Ab: 1ug/ml Rabbit Anti-Human Smad3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: Smad3 knockout PC3 cell lysate; Predicted MW: 26,36,43,48kd Observed MW: 43kd Primary Ab: 1ug/ml Rabbit Anti-Human Smad3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
Uniprot Description
SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor
Chromosomal Location of Human Ortholog: 15q22.33
Cellular Component: cytoplasm; cytosol; nuclear chromatin; nuclear inner membrane; nucleoplasm; nucleus; plasma membrane; receptor complex; SMAD protein complex; SMAD2-SMAD3 protein complex; transcription factor complex
Molecular Function: beta-catenin binding; bHLH transcription factor binding; chromatin DNA binding; co-SMAD binding; collagen binding; DEAD/H-box RNA helicase binding; DNA binding transcription factor activity; glucocorticoid receptor binding; identical protein binding; mineralocorticoid receptor binding; phosphatase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; R-SMAD binding; sequence-specific DNA binding; transcription factor binding; transcription regulatory region DNA binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding
Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; activin receptor signaling pathway; cell cycle arrest; cell-cell junction organization; cellular response to transforming growth factor beta stimulus; developmental growth; embryonic cranial skeleton morphogenesis; embryonic foregut morphogenesis; embryonic pattern specification; endoderm development; evasion or tolerance of host defenses by virus; heart looping; immune response; immune system development; in utero embryonic development; lens fiber cell differentiation; liver development; mesoderm formation; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of lung blood pressure; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; nodal signaling pathway; osteoblast development; paraxial mesoderm morphogenesis; pericardium development; positive regulation of alkaline phosphatase activity; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of chondrocyte differentiation; positive regulation of epithelial to mesenchymal transition; positive regulation of extracellular matrix assembly; positive regulation of focal adhesion assembly; positive regulation of gene expression; positive regulation of interleukin-1 beta production; positive regulation of nitric oxide biosynthetic process; positive regulation of positive chemotaxis; positive regulation of stress fiber formation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of transforming growth factor beta3 production; primary microRNA processing; protein deubiquitination; protein stabilization; reduction of cytosolic calcium ion concentration; regulation of binding; regulation of epithelial cell proliferation; regulation of immune response; regulation of striated muscle tissue development; regulation of transcription from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; SMAD protein complex assembly; somitogenesis; T cell activation; thyroid gland development; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; ureteric bud development; Wnt receptor signaling pathway through beta-catenin; wound healing
Disease: Loeys-dietz Syndrome 3
Research Articles on Smad3
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Product Notes
The Smad3 smad3 (Catalog #AAA2003140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mothers Against Decapentaplegic Homolog 3 (Smad3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Smad3 smad3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- FWCSISYYEL NQRVGETFHA SQPSMTVDGF TDPSNSERFC LGLLSNVNRN AAVELTRRHI GRGVRLYYIG GEVFAECLSD SAIFVQSPNC NQRYGWHPAT VCKIPPGCNL KIFNNQEFAA LLAQSVNQGF EAVYQLTRMC TIRMSFVKGW GAEYRRQTVT. It is sometimes possible for the material contained within the vial of "Mothers Against Decapentaplegic Homolog 3 (Smad3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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