Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Supervillin (SVIL) Polyclonal Antibody | anti-SVIL antibody
Polyclonal Antibody to Supervillin (SVIL)
MGHHHHHHSGSEF-CVYFFW QGRHSTVSEK GTSALMTVEL DEERGAQVQV LQGKEPPCFL QCFQGGMVVH SGRREEEEEN VQSEWRLYCV RGEVPVEGNL LEVACHCSSL RSRTSMVVLN VNKALIYLWH GCKAQAHTKE VGRTAANKIK EQCPL
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach cancer tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach cancer tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Several transcript variants encoding different isoforms of supervillin have been described. [provided by RefSeq, Apr 2016]
Uniprot Description
supervillin: an actin-binding protein that links filamentous actin to the plasma membrane. Binds filamentous actin and myosin II bind within the rsds 1-830 of the amino terminus, an extended intrinsically disordered region (IDR). Forms a high-affinity link between the actin cytoskeleton and the plasma membrane. Four human isoforms are formed by alternative splicing. Isoform 1 (archvillin) is muscle specific and among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6.
Protein type: Actin-binding; Contractile; Motility/polarity/chemotaxis; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 10p11.23
Cellular Component: actin cytoskeleton; cleavage furrow; costamere; cytoplasm; cytosol; focal adhesion; invadopodium; microtubule minus-end; midbody; nucleus; plasma membrane; podosome
Molecular Function: actin filament binding; protein binding
Biological Process: cytoskeleton organization; positive regulation of cytokinesis; skeletal muscle tissue development
Research Articles on SVIL
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Product Notes
The SVIL svil (Catalog #AAA2002837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Supervillin (SVIL) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Supervillin (SVIL) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SVIL svil for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-CVYFFW QGRHSTVSEK GTSALMTVEL DEERGAQVQV LQGKEPPCFL QCFQGGMVVH SGRREEEEEN VQSEWRLYCV RGEVPVEGNL LEVACHCSSL RSRTSMVVLN VNKALIYLWH GCKAQAHTKE VGRTAANKIK EQCPL. It is sometimes possible for the material contained within the vial of "Supervillin (SVIL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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