Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Spleen Tissue; Lane2: Mouse Stomach Tissue; Lane3: Human Raji Cells.)
Rabbit anti-Mouse Nuclear Factor Kappa B (NFkB) Polyclonal Antibody | anti-NFkB antibody
Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- P YLQILEQPKQ RGFRFRYVCE GPSHGGLPGA SSEKNKKSYP QVKICNYVGP AKVIVQLVTN GKNIHLHAHS LVGKHCEDGV CTVTAGPKDM VVGFANLGIL HVTKKKVFET LEARMTEACI RGYNPGLLVH SDLAYLQAEG GGDRQLTDRE KEIIRQAAVQ QTKEMDLSVV RLMFTAFLPD STGSFTRRLE PVVSDAIYDS KAPNASNLKI VRMDRTAGCV TGGEEIYLLC DKVQKDDIQI RFYEEEENGG VWEGFGDFSP TDVHRQFAIV FKTPKYKDVN ITKPASVFVQ LRRKSDLETS EPKPFLYYPE IKDKEEVQRK
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Spleen Tissue; Lane2: Mouse Stomach Tissue; Lane3: Human Raji Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Spleen Tissue; Lane2: Mouse Stomach Tissue; Lane3: Human Raji Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NFkB, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NFkB, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
Uniprot Description
NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 3 G3|3 62.82 cM
Cellular Component: cytoplasm; cytosol; mitochondrion; neuron projection; nucleoplasm; nucleus; protein complex
Molecular Function: actinin binding; chromatin binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; heat shock protein binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription regulatory region DNA binding
Biological Process: apoptosis; cellular response to brain-derived neurotrophic factor stimulus; cellular response to carbohydrate stimulus; cellular response to cytokine stimulus; cellular response to diterpene; cellular response to dsRNA; cellular response to glucoside; cellular response to interleukin-1; cellular response to interleukin-6; cellular response to nicotine; cellular response to organic cyclic compound; cellular response to peptide; cellular response to peptide hormone stimulus; cellular response to tumor necrosis factor; I-kappaB kinase/NF-kappaB signaling; innate immune response; lymph node development; negative regulation of apoptosis; negative regulation of calcidiol 1-monooxygenase activity; negative regulation of cytokine production; negative regulation of gene expression; negative regulation of inflammatory response; negative regulation of interleukin-12 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; NIK/NF-kappaB signaling; positive regulation of canonical Wnt signaling pathway; positive regulation of gene expression; positive regulation of gene silencing by miRNA; positive regulation of hyaluronan biosynthetic process; positive regulation of miRNA metabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to cytokine; response to muscle stretch; response to organic cyclic compound; response to oxidative stress; signal transduction; transcription, DNA-dependent
Research Articles on NFkB
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Product Notes
The NFkB nfkb1 (Catalog #AAA2002452) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nuclear Factor Kappa B (NFkB) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear Factor Kappa B (NFkB) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NFkB nfkb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- P YLQILEQPKQ RGFRFRYVCE GPSHGGLPGA SSEKNKKSYP QVKICNYVGP AKVIVQLVTN GKNIHLHAHS LVGKHCEDGV CTVTAGPKDM VVGFANLGIL HVTKKKVFET LEARMTEACI RGYNPGLLVH SDLAYLQAEG GGDRQLTDRE KEIIRQAAVQ QTKEMDLSVV RLMFTAFLPD STGSFTRRLE PVVSDAIYDS KAPNASNLKI VRMDRTAGCV TGGEEIYLLC DKVQKDDIQI RFYEEEENGG VWEGFGDFSP TDVHRQFAIV FKTPKYKDVN ITKPASVFVQ LRRKSDLETS EPKPFLYYPE IKDKEEVQRK. It is sometimes possible for the material contained within the vial of "Nuclear Factor Kappa B (NFkB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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