Rabbit Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) Polyclonal Antibody | anti-MUSK antibody

Polyclonal Antibody to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)

Cat. Num. AAA2002046

• Gene Names: Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• Reactivity: Mus musculus (Mouse)
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Muscle; Skeletal; Receptor Tyrosine Kinase (MUSK); Polyclonal Antibody; Polyclonal Antibody to Muscle; anti-MUSK antibody

• Host: Rabbit
• Reactivity: Mus musculus (Mouse)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 0.43mg/ml (varies by lot)

• Applicable Applications for anti-MUSK antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunoprecipitation (IP).
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Human; Rat
• Immunogen: Recombinant MUSK (Cys517~Arg777 (Accession # Q61006)) expressed in E.coli (MBS2018741).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Human Jurkat Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant MUSK, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Heart Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse MUSK AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )

NCBI and Uniprot Product Information

• NCBI GI #: 82533041
• NCBI GeneID: 18198
• NCBI Accession #: NP_001032205.1
• NCBI GenBank Nucleotide #: NM_001037128.1
• UniProt Accession #: Q61006; Q61005; Q61987; Q61988
• NCBI Official Full Name: muscle, skeletal receptor tyrosine-protein kinase isoform 2
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: Musk
• NCBI Official Synonym Symbols: Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muscle, skeletal receptor tyrosine protein kinase
• UniProt Gene Name: Musk
• UniProt Synonym Gene Names: Nsk2; MuSK; Muscle-specific kinase receptor

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Musk family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 4 B3|4 31.87 cM

Cellular Component: cell junction; cell projection; external side of plasma membrane; integral component of membrane; integral component of plasma membrane; membrane; neuromuscular junction; plasma membrane; postsynaptic membrane; receptor complex; synapse

Molecular Function: ATP binding; kinase activity; metal ion binding; nucleotide binding; PDZ domain binding; protein binding; protein kinase activity; protein kinase binding; protein tyrosine kinase activity; transferase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell differentiation; memory; multicellular organism development; negative regulation of gene expression; neuromuscular junction development; phosphorylation; positive regulation of gene expression; positive regulation of neuron apoptotic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein geranylgeranylation; positive regulation of protein phosphorylation; positive regulation of skeletal muscle acetylcholine-gated channel clustering; positive regulation of synaptic growth at neuromuscular junction; positive regulation of synaptic transmission, cholinergic; protein amino acid phosphorylation; protein autophosphorylation; receptor clustering; regulation of synaptic growth at neuromuscular junction; regulation of transcription, DNA-templated; response to electrical stimulus; skeletal muscle acetylcholine-gated channel clustering